番茄多聚半乳糖醛酸酶PG反义基因表达及相关功能研究

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1、中国农业大学博士学位论文番茄多聚半乳糖醛酸酶（PG）反义基因表达及相关功能研究姓名：寇晓虹申请学位级别：博士专业：食品科学指导教师：罗云波20030501AbstractPolygalacturonase(PG)playsmajorrolesinfruitsoftening，organabscission，pollinationandseeddevelopment．Therelationshipbetweenpolygalacturonaseandethylene，otherplanthormone，andtheposi

2、tionsofPGinplantresistanceanddiseasesresponseswerespeciallydiscussedinthispaper．ThemultiformityofPGfunctionswasindicated．Agrobacterium·-mediatedtransformationhadbeenusedtointroducetheAnti-PGgeneintoprocessingtomato(cv．98—75)．Fourpositivetransformedtomatoplantswer

3、econfirmedbyPCRandSouthernhybridization．Fruitfirmnessandsolublesolidoftransgenictomatowerehigherthanthoseofcontrol；fruitwerefullandlustrous；solublepectinwaslowerthancontrolatthesamestage．ThePGactivityoftransgenictomatowas83％，88％，63％，46％ofcontroltomatorespectively

4、NorthernhybridizationshowedthatallPG，LOgandLeEXPlmRNAdecreasedintransgenictomatofruitcomparedwiththewildtomato．Theseresultssuggestthattheprocessofripeningandsofteningintransgenicanti-PGtomatoweredelayed，TheexpressionpatternsofPG．LeEXPlandLOXwerestudiedinwildtomat

5、o，antisenseACStomatoandNrmutanttomato．TheresultshowedthatPGplayedamajorroleinripeningandsofteningoftomatofruit,andcooperatedwiththeregulationofLeEXPlandLOX．TherelationshipbetweenathyleneandPolygalacturonase俨(甜Wasthoroughlyinvestigatedintwoaspectsoftheeffectofethy

6、leneonPGfunctionandtheregulationofPGonethylenesynthesis．Withwildtomato，antisenseACStomato，Nrmutanttomatoandwildtomatotreatedwithexogenousethyleneand1-MCPasmaterials，itwasshowedthattheexpressionofPGgeneandPGactivitywereinhibitedsignificantlyinantisenseACStomatoand

7、Nrmutanttomato．TheexpressionofPGwaspromotedwithexogenousethylene(100p．1／L)andobviouslyinhibitedwithl—MCP(1I_tl／L)．TheseresultssuggestedthatexpressionofPGgenewasregulatedbyethylene．Ontheotherhand，withantisensePGtomatoandwildtomatotreatedwith100mg／LD-GAasmaterials，

8、itwasshowedthattheethyleneproductionofantisensePGtomatowaslowerthanthatofwildtomato，theexpressionofethylenereceptorgeneLeETR4andtheethyleneresponsefactorLeERF2