重组酶介导等温核酸扩增技术快速检测烟曲霉菌

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1、分类号_______________论文编号.______________密级.硕士学位论文重组酶介导等温核酸扩增技术快速检测烟曲霉菌RapiddetectionofAspergillusfumigatuswithRecombinasePolymeraseAmplificationAssay研究生姓名：廖新辉学号：20111389指导教师：兰小鹏、主任技师、福州总医院检验科学科、专业：__________临床检验诊断学_______________学位类型：___________科学学位________________答辩日期：______

2、_____2015年05月________________二〇一五年五月独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研宄工作。除了文中特别加以标注和致谢的地方外，论文不包含其它人已经发表或撰写过的研宄成果。与我一同工作的同志对本研究所做的任何贡献均已在论文中明确的说明并表示谢意。本人承担本声明的法律责任。学位论文版权使用授权声明本人完全了解第二军医大学有关保留、使用学位论文的规定，第二军医大学有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权第二军医大学可以将学位论文全文或部分内容编入《中

3、国学位论文全文数据库》、《中国优秀博硕士学位论文全文数据库》等数据库进行检索。可以采用影印、缩印或扫描等复制手段保存、汇编学位论文。（保密的学位论文在解密后适用本授权书}年才月/沪曰羝少1T年J月听第二军医大学硕士学位论文重组酶介导等温核酸扩增技术快速检测烟曲霉菌RapiddetectionofAspergillusfumigatuswithRecombinasePolymeraseAmplificationAssay研究生姓名：廖新辉____________学号：20111389指导教师：兰小鹏、主任技师、福州总医院检验科学科、专业:__

4、____________临床枪验诊断学______________学位类型:___________________科学学位__________________培养单位:___________南京军区福州总医院检验科二〇一五年五月重组酶介导的等温核酸扩增技术快速检测烟曲霉目录中文摘要···············································································1英文摘要················································

5、·······························3缩略词表···············································································5正文···············································································6一、前言·····························································6二、资料与方法··

6、·························································10三、结果···························································20四、讨论·························································27五、参考文献·························································34综述···················

7、·····················································39在校期间发表论文·································································50致谢···················································································51重组酶介导的等温核酸扩增技术快速检测烟曲霉摘要【目的】以烟曲霉内转录间隔区（ITSD）为靶序列，筛选烟曲霉菌特异

8、性的引物对，应用重组酶介导的等温核酸扩增(RPA)技术建立快速检测烟曲霉的新方法并对该检测方法进行方法学优化。【方法】（1）以烟曲霉菌的易变区内转录间隔区为靶序列，先用Prime