弓形虫抑制wntβ-catenin通路干扰c17.2神经干细胞的分化

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1、分类号：________________________密级：_________________________UDC：一__编号：-______一一_____：学位论文弓形虫抑制Wnt/p-catenin通路干扰C17.2神经干细胞的分化ToxoplasmagondiiinhibitsthedifferentiationofCl7.2neuralstemcellsthroughWnt/(3-cateninPathway甘小凤指导教师姓名余莉教授安徽医科大学微生物学教研室申请学位级别硕士专业名称微生物学提交论文日期2015-

2、03论文答辩曰期2015-05学位授予单位和日期安徽医科大学2015-07次■办.答辩委员会主席龚镇奎评阅人2015年05月学位论文原创性声明本人所呈交的论文是我个人在导师指导下进行的研宄工作及取得的研宄成果。据我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人己经发表或撰写过的研宄成果。与我一同工作的同志对本研究所做的任何贡献均己在论文中作了明确说明并表示谢意。学位论文作者签名：日期：学位论文授权使用声明本人完全了解安徽医科大学有关保留、使用学位论文的规定：学校有权保留学位论文并向国家主管部门或其指定机构送交论

3、文的电子版和纸质版，有权允许论文进入学校图书馆被查阅，有权将学位论文的内容编入有关数据库进行检索，有权将学位论文的标题和摘要汇编出版。愿意将本人的学位论文提交《中国博士学位论文全文数据库》、《中国优秀硕士学位论文全文数据库》和《中国学位论文全文数据库》中全文发表，并可以以电子、网络及其他数字媒体形式公开出版，并同意编入CNKI《中国知识资源总库》，在《中国博硕士学位论文评价数据库》中使用和在互联网上传播。保密的学位论文在解密后适用本规定。学位论文作者签名:导师签名：f•慕日期：曰期可安徽医科大学UNHUIMEDICALUNI

4、VERSITY硕士学位论文弓形虫抑制Wnt/β-catenin通路干扰C17.2神经干细胞的分化ToxoplasmagondiiinhibitsthedifferentiationofC17.2neuralstemcellsthroughWnt/β-cateninPathway作者姓名：甘小凤指导教师：余莉教授学科（专业）：微生物学申请学位类别：硕士论文工作时间：2013.03-2015.032015年5月目录中英文缩略词对照··················································

5、··············1中文摘要············································································2英文摘要············································································41前言···············································································62材料与方

6、法······································································92.1实验材料···································································92.1.1实验动物····························································92.1.2实验虫株·······································

7、·····················92.1.3实验细胞····························································92.1.4实验主要试剂······················································92.1.5实验主要试剂的配置···········································102.1.6实验主要仪器·····································

8、···············122.2实验方法·································································132.2.1弓形虫在小鼠体内的复苏、传代、收集和冻存··········132.2.2C17.2神经干细胞的复