基于中药方剂配伍机理的中药有效成分在食管癌前病变进程中的免疫调节作用

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1、授予单位代码10089学号或申请号12408HebeiMedicalUniversity硕士学位论文在职科学学位基于中药方剂配伍机理的中药有效成分在食管癌前病变进程中的免疫调节作用学位申请人：韩利霞导师：单保恩教授王玲副教授专业：免疫学2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下,由本人独立完成。本学位论文研究所获得的研究成果,其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公开和使用。凡发表与学位论文主要内容相关的论文,

2、第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所有。否则,承担相应法律责任。研究生签名导师签章^^二级学院领导盖章：^年月日河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果,除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果,指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名：本冽久导师签章：巧…也年^月夕曰中文摘要目录中文摘要··························

3、·········································1英文摘要································································3研究论文基于中药方剂配伍机理的中药有效成分在食管癌前病变进程中的免疫调节作用前言·······································································6材料与方法······················

4、····································7结果·································································11附图··································································14附表········································································

5、·17讨论···································································21结论·········································································24参考文献······································································25综述食管癌诊疗概况和分子水平研究进展·····

6、·····························27致谢········································································33个人简历···········································································35中文摘要基于中药方剂配伍机理的中药有效成分在食管癌前病变进程中的免疫调节作用摘要目的：本课题拟研究“连翘、天花粉蛋白、党参、

7、香加皮”中有效成分组成的“连花参加”方逆转化学致癌剂4-硝基喹啉-1-氧化物（4-nitro-quinoline-1-oxide，4NQO）诱导小鼠(C57BL/6)食管癌前病变的效果及潜在的免疫调节机制。方法：1选取300只C57BL小鼠动物模型，按随机原则分为6组，分别为空白对照组（A1组）（不做任何处理）30只；甲基纤维素对照组（A2组）30只，给予甲基纤维素灌胃，不给药；诱癌剂组(B组)，治疗组（C组），预防组（D组），全反式维甲酸阳性对照组（E组），用灭菌水配制成100μg/ml浓度，

8、采取正常饮水法给B、C、D、E四组小鼠。受试药“连花参加”方的配制方法为连翘脂素(2mg):天花粉蛋白（1.4mg）:香加皮三萜类化合物（1.4mg）:党参蛋白（0.7mg），按此比例混合溶于1.5%的甲基纤维素，定容20ml。D组在实验开始就给与中药方剂“连花参加”方灌胃。C组第15周开始给予中药方剂“连花参加”方灌胃，E组也在15周进行“全反式维甲酸（ATRA）”灌胃。C、D、E组小鼠每周均灌胃三次，每次0.1ml。2该实验从第12周时分别把A1、A2、B、D组小鼠处死5只，观察结果显示小鼠