differentially activation of tlr3 by very virulent and attenuateg virulent infectious bursal disease virus in the bursa of fabricuis in commercial chickens论文

《differentially activation of tlr3 by very virulent and attenuateg virulent infectious bursal disease virus in the bursa of fabricuis in commercial chickens论文》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、学校代码10608学号L201207101001分类号S855.3密级公幵GuangxiUniversityforNationalities碩士学位办丈DifTerentiallyactivationofTLR3byveryvirulentandattenuatedvirulentInfectiousBursalDiseasevirusinthebursaofFabricuisincommercialchickens研究生姓名：康思那导师姓名职称：何秀苗教授学科专业：生物化学与分子生物学所属学院：海洋与生物技术学院年级：2

2、012级论文完成时间：201S年6月࠶㊫ਧ˖S855.3ᇶ㓗˖ޜᔰ⺅༛⹄ウ⭏ᆖս䇪᮷䇪᮷仈ⴞ˖DifferentiallyactivationofTLR3byveryvirulentandattenuatedvirulentInfectiousBursalDiseasevirusinthebursaofFabricuisincommercialchickensуъ˖⭏⢙ॆᆖо࠶ᆀ⭏⢙ᆖ⹄ウᯩੁ˖⯵∂࠶ᆀ⭏⢙ᆖ⹄ウ⭏˖ᓧᙍ䛓ᤷሬ㘱ᐸ˖օ⿰㤇ᮉᦸ䇪᮷䎧→ᰕᵏ2013ᒤ4ᴸ㠣2015ᒤ5ᴸDifferentiallyactiva

3、tionofTLR3byveryvirulentandattenuatedvirulentInfectiousBursalDiseasevirusinthebursaofFabricuisincommercialchickensAbstractToll-likeReceptor3(TLR3)isoneoftheTLRswhoseligandisdoublestrandRNA(dsRNA).Infectiousbursaldiseasevirus(IBDV),whichisadsRNAvirus,couldberecogni

4、zedbyTLR3.InfectiousBursalDisease(IBD)causessignificantlossestothepoultryindustries,suchashighmortality,andimmunosuppressioninyoungchickens.Uptonow,littleisknownonthesignalingpathway,mechanisms,anddeterminesthefunctionofchTLR3onIBDVinfectioninvivo.Inthisstudy,itis

5、importantandessentialtostudytherelationshipofChTLR3signalingpathwaywithIBDV-infectionincommercialchickens.Aspreviously,ourgroupwassuccessfullycloningchicken¶stolllike-receptor3gene(GX-sh-chTLR3).Inthisstudy,thepolyclonalantibodyagainstChTLR3waspreparedbyimmunizati

6、onofBALB/cmicewithGX-sh-chTLR3protein,whichwasexpressedinE-coli.AnIndirectEnzymeLink-immunosorbentAssay(ELISA)wasdevelopedfordetectionoftheantibodylevelinmiceserum.Theresultsshowedthatamongvariousserumdilution,1:800immuneserumdilutionwith1:400antigen(chTLR3-LRRrec

7、ombinantprotein)dilutionarethebestconditionforELISAdetection,whereas1:100immuneserumdilutionwith1:400immuneserumdilutionshowedthelowestpositive(2.21ratio).ThisresultsuggeststhatthepolyclonalantibodypreparedinthisstudyhasahighsensitivityfordetectingofrecombinantchT

8、LR3-LRRbyELISA.TherelationshipofchTLR3andIBDVinfectionwascarriedoutinnon-vaccinated4-week-oldcommercialchickensbychallengingwithvvIBDVstrainNN1172andatt