糖尿病来源的骨髓间充质干细胞治疗心肌梗死的相关研究

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1、分类号R654.2第四军医大学UDC617.1TheFourthMilitaryMedicalUniversity密级公开博士学位论文糖尿病来源的骨髓间充质干细胞治疗心肌梗死的相关研究刘宇培养类别全日制学位类型学术学位一级学科(专业类)临床医学二级学科(专业)外科学（心胸外科）研究方向干细胞移植治疗心肌梗死指导教师王辉山教授（主任医师）培养单位西京医院心血管外科二O一五年五月第四军医大学博士学位论文目录缩略语表··················································································

2、·······················1中文摘要·········································································································3英文摘要·········································································································6前言···········································

3、····························································10文献回顾·······································································································12正文·······································································································20第一部分糖尿病与非糖尿

4、病患者骨髓间充质干细胞基因表达差异分析···················201材料····································································································202方法····································································································232.1MSC的获取、分离、培养························

5、··········································232.2MSC生长曲线的绘制········································································242.3MSC的形态学和表型鉴定··································································242.4基因芯片检测MSC基因表达···························································

6、···242.5基因表达差异在蛋白水平的验证··························································302.6统计学处理·····················································································313结果····································································································313.1MSC的

7、培养和观察········································································313.2MSC表面标志物鉴定········································································333.3基因芯片待测RNA质量控制情况························································333.4基因表达谱差异情况············