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ID:34954920
大小:57.50 KB
页数:11页
时间:2019-03-15
《蛋白与核酸的分析工具http》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、...页眉1.蛋白及核酸分析工具http://www.expasy.org/tools/是一个强大的集成生物信息网站。(1)此网页的包括了预测拓扑Topologyprediction•NetNESLeucine-richnuclearexportsignals(NES)ineukaryoticproteins(真核蛋白的富含亮氨酸的核输出信号)•PSORT-Predictionofproteinsubcellularlocalization(蛋白亚细胞定位)•SecretomeP:Non-classicalandleaderlesssecretionofproteins(蛋白的非
2、经典和无导肽分泌)•TargetP-Predictionofsubcellularlocation(亚细胞定位)•TatP-Twin-argininesignalpeptides(精氨酸信号肽)•DAS-PredictionoftransmembraneregionsinprokaryotesusingtheDenseAlignmentSurfacemethod(StockholmUniversity)(原核生物中跨膜区预测)•HMMTOP-Predictionoftransmembranehelicesandtopologyofproteins(HungarianAcademy
3、ofSciences)(蛋白跨膜螺旋和拓扑结构预测)•PredictProtein-Predictionoftransmembranehelixlocationandtopology(ColumbiaUniversity)(跨膜螺旋定位和拓扑预测)•SOSUI-Predictionoftransmembraneregions(NagoyaUniversity,Japan)(跨膜区预测)•TMHMM-Predictionoftransmembranehelicesinproteins(CBS;Denmark)(跨膜区螺旋预测)•TMpred-Predictionoftransmem
4、braneregionsandproteinorientation(EMBnet-CH)(跨膜区和蛋白定向预测)•TopPred-Topologypredictionofmembraneproteins(France)(膜蛋白的拓扑预测)最常用的跨膜区预测的两个网站预测跨膜螺旋结构的TMHMM-Predictionoftransmembranehelicesinproteins(CBS;Denmark)网页为http://www.cbs.dtu.dk/services/TMHMM-2.0/预测跨膜区以及跨膜方向的TMpred-Predictionoftransmembranere
5、gionsandproteinorientationhttp://www.ch.embnet.org/software/TMPRED_form.html....页脚...页眉(2)蛋白质一级结构预测Primarystructureanalysis•ProtParam-Physico-chemicalparametersofaproteinsequence(amino-acidandatomiccompositions,isoelectricpoint,extinctioncoefficient,etc.)(蛋白序列的化学参数包括氨基酸、原子组成、等电电、消光系数等)•Comput
6、epI/Mw-Computethetheoreticalisoelectricpoint(pI)andmolecularweight(Mw)fromaUniProtKnowledgebaseentryorforausersequence(计算理论上的等电点和分子重)•ScanSitepI/Mw-ComputethetheoreticalpIandMw,andmultiplephosphorylationstates(计算理论上的等电点和分子重和多磷酸化状态)•MW,pI,Titrationcurve-ComputespI,compositionandallowstoseeatit
7、rationcurve(计算理论上的等电点和组成,可以看滴定曲线)•ScratchProteinPredictor•HeliQuest-Awebservertoscreensequenceswithspecificalpha-helicalproperties(一个网站利用专门的alpha螺旋扫描蛋白)•Radar-Denovorepeatdetectioninproteinsequences(蛋白序列的重头重复检测)•REP-Searchesaproteinsequencefo
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