粘细菌胞外多糖提取纯化及其生物活性检测 毕业论文

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1、粘细菌胞外多糖提取纯化及其生物活性检测装订线摘　　要　　随着这几年来糖化学的迅速发展，微生物胞外多糖的理论和应用也受到了人们的重视。胞外多糖的提取纯化和组分分析的实验技术都已成熟，为本实验提供了理论基础和实验指导。本文通过观察粘细菌在CAS固体培养基平板上的菌落黏性圈，从79株粘细菌中筛选到6株胞外多糖产量高的菌株，并对其中4株粘细菌产生的胞外多糖进行了提取纯化。粘细菌经液体发酵培养后，将发酵液进行乙醇沉淀得到粗多糖的提取物。粗多糖经氯仿、三氯乙酸脱蛋白，过氧化氢脱色，得到白色粉状纯化物。纯化的粘细菌胞外多糖采用0.5mol/L

2、盐酸水解后，经高效液相色谱分析，可确定菌株92033、93147、94016的胞外多糖中均含有葡萄糖、甘露糖，其他成分需进一步验证。4株粘细菌的胞外多糖纯化物的水溶液，经絮凝活性检测实验表明，均具有絮凝活性，其中菌株92033对4g/L的高岭土悬浊液的絮凝率达到92.84%，显示出良好的应用前景。关键词：糖化学　　胞外多糖　　粘细菌　　絮凝活性ABSTRACTAstherapiddevelopmentofsugarchemicaloverthepastfewyears,thetheoryandapplicationofthemi

3、crobialexopolysaccharideswereimportantforthepeople.Theextraction,purificationandcomponentanalysisexperimentaltechniqueoftheexopolysaccharidehadbeenmature,whichprovidedatheoreticalbasisandexperimentalguidance.Inthisarticle,Myxobacteriawhichproducedmanypolysaccharidewe

4、rescreenedfromtheCASsolidmediumbyobservingthestickinessofcolony.Wehadgotsixstrainswhichcouldproducedhighexopolysaccharidefromthe79strains,andfourstrainshadbeenextractedandpurificated.Afterliquidculturefermentation,wecouldobtainedthecrudepolysaccharideextractsbyalcoho

5、lprecipitationmethdfromthefermentationbroth.Weobtainedthewhitepowderofthepolysaccharidebythechloroformandtrichloroaceticacidremovingproteinandhydrogenperoxidedetreated.AfterpurifiedMyxobacteriaexopolysaccharides0.5mol/Lhydrochloridehydrolysis,wecoulddeterminethatthes

6、trainsof92033,93147,94016whichcontainingglucoseandmannosebyhighperformanceliquidchromatographyanalysis.Othercomponentswereprovedbyfurthervalidation.Afterthetestingoftheflocculatingactivity,thefourmyxobacteriaaqueoussolutionofexopolysaccharidepurificationwereallhadflo

7、cculationactivity.Strainsofthe92033hadaneffcton4g/Lkaolinsuspensionandtheflocculationraterichto92.84%anditshowedagoodapplicationprospect.Keywords:sugarchemical　　exopolysaccharides　　Myxobacteria　　flocculationactivity目　　录一前言…………………………………………………………………11.1粘细菌简介……………………………

8、…………………………………11.2微生物胞外多糖概况……………………………………………………11.3微生物胞外多糖的应用…………………………………………………21.3.1胞外多糖的药用性……………………………………………………21.3.2胞外多糖的絮凝性…………