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ID：34913904
大小：9.75 MB
页数：58页
时间：2019-03-14
《平喘宁调节erk信号通路相关蛋白干预哮喘气道重塑的效应机制研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、单位代码：10369学'号：201200122015届硕士研究生学位论文癱平喘宁调节ERK信号通路相关蛋白千预哮喘气道重塑的效应机制研究RESEARCHONTHEEFFECT3VIECHANISMOFASTHMAAIRWAYREMODELINGOFERKSIGNALINGPATHWAYRELATEDPROTEINSREGULATEOFPINGCHUANNING学科专业:中医方剂学研究方向:方荆K伍与作用规律研究导师:方向明教授碩士生：__________刘韹论文完成单位：安徽中医药大学22、相关蛋白干预哮喘气道重塑的效应机制研究ResearchontheeffectmechanismofasthmaairwayremodelingofERKsignalingPathwayrelatedProteinsregulateofPingchuanning作者姓名：刘静申请学位级别：医学科学硕士学位指导教师姓名：方向明职称：教授学科专业：中医方剂学研究方向：方剂配伍与作用规律研究学习时间自：2012年09月01日起至2015年06月21日止论文提交日期：2015年03月01日论文答辩日期：2015年06月04日学位授予单位：安徽中医药大学学位类型：医学科学硕士学位学位论文原创性声3、明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进行研究工作所取得的成果。除文中已经注明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。学位论文作者（需亲笔）签名：列衝《月'曰学位论文版权使用授权书本人完全了解安徽中医药大学有关保留、使用学位论文的规定，同意学校保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被査阅和借阅。本人授权安徽中医药大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段4、保存和汇编本学位论文。保密口，在_年解密后适用本授权书。本学位论文属于不保密口。(请在以上方框内打“V”）学位论文作者（需亲笔）签名：o/W年/月/曰导师（需亲笔）签名：年〈月I日目录目录中文摘要············································3英文摘要······································5英文缩略词表·································7正文1.前言·······································82.实验研究·················5、······················92.1实验动物饲养与分组························92.2实验试剂与仪器··································112.3实验药物与造模·······························122.4实验取材与标本保存·······························132.5实验统计方法····································133.实验检测·········································143.1标本6、固定与石蜡切片制作···························143.2HE染色原理与步骤································153.3免疫组化原理与步骤·······························153.4Western-Blot法原理与实验步骤····················174.实验结果·········································214.1HE检测结果······································214.2免疫组化检测MMP-9、TIMP-1结果7、·················224.3Western-Blot法检测PDGF、RAS、MEK1、P-ERK1、ERK1、C-FOS结果··········································255.讨论··············································275.1哮喘相关研究································275.1.1哮喘模型研究··········
2、相关蛋白干预哮喘气道重塑的效应机制研究ResearchontheeffectmechanismofasthmaairwayremodelingofERKsignalingPathwayrelatedProteinsregulateofPingchuanning作者姓名：刘静申请学位级别：医学科学硕士学位指导教师姓名：方向明职称：教授学科专业：中医方剂学研究方向：方剂配伍与作用规律研究学习时间自：2012年09月01日起至2015年06月21日止论文提交日期：2015年03月01日论文答辩日期：2015年06月04日学位授予单位：安徽中医药大学学位类型：医学科学硕士学位学位论文原创性声
3、明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进行研究工作所取得的成果。除文中已经注明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重要贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。学位论文作者（需亲笔）签名：列衝《月'曰学位论文版权使用授权书本人完全了解安徽中医药大学有关保留、使用学位论文的规定，同意学校保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被査阅和借阅。本人授权安徽中医药大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段
4、保存和汇编本学位论文。保密口，在_年解密后适用本授权书。本学位论文属于不保密口。(请在以上方框内打“V”）学位论文作者（需亲笔）签名：o/W年/月/曰导师（需亲笔）签名：年〈月I日目录目录中文摘要············································3英文摘要······································5英文缩略词表·································7正文1.前言·······································82.实验研究·················
5、······················92.1实验动物饲养与分组························92.2实验试剂与仪器··································112.3实验药物与造模·······························122.4实验取材与标本保存·······························132.5实验统计方法····································133.实验检测·········································143.1标本
6、固定与石蜡切片制作···························143.2HE染色原理与步骤································153.3免疫组化原理与步骤·······························153.4Western-Blot法原理与实验步骤····················174.实验结果·········································214.1HE检测结果······································214.2免疫组化检测MMP-9、TIMP-1结果
7、·················224.3Western-Blot法检测PDGF、RAS、MEK1、P-ERK1、ERK1、C-FOS结果··········································255.讨论··············································275.1哮喘相关研究································275.1.1哮喘模型研究··········
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