云南省楚雄州蚊虫及蚊媒病毒调查研究

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1、12缩略词表英文缩写英文全名中文译名RT-PCRreversetranscription-polymerasechainreaction逆转录-聚合酶链反应JEVJapaneseencephalitisvirus流行性乙型脑炎病毒SINVSindbisvirus辛德毕斯病毒CPEcytopathiceffect细胞病变效应CHIKVChikungunyavirus基孔肯雅病毒GETVGetahvirus盖塔病毒DENVDenguevirus登革病毒YUOVYunnanorbivirus云南环状病毒KDVKadiprovirusKadipiro病毒BAVBannavirus版

2、纳病毒BATVBataivirus巴泰病毒MIRMinimuminfectionrate最低感染率ICTVInternationalCommitteeonTaxonomyofViruses国际病毒分类委员会CxFVCulexflavivirus库蚊黄病毒RdRpRNA-dependentRNApolymeraseRNA依赖的RNA聚合酶ORFopenreadingframe开放读码框架NDiVNamDinhvirusNamDinh病毒3目录中文摘要································································

3、·········2英文摘要·········································································3前言··············································································6内容与方法·····································································9结果············································

4、·································14讨论·············································································20结论·············································································22参考文献········································································23附录·············

5、································································27文献综述········································································304云南省楚雄州蚊虫及蚊媒病毒调查研究研究生：杨晓羽导师：周红宁教授摘要目的：研究出云南楚雄元谋、武定和双柏3县蚊虫种类组成及其主要虫媒病毒种类，为制定出有效的虫媒传染病防控对策提供依据。方法：在楚雄元谋、武定和双柏3县，根据不同纬度、经度和海拔,每县选取两个具有代表性的蚊虫调查点并采用GPS

6、收集调查点相关数据。（1）采用诱蚊灯对调查点人、牛、猪房通宵捕捉成蚊，采用双层叠帐从早上8:00至晚上20:00捕捉白天活动的蚊虫，在调查点周围蚊虫孳生环境采集蚊虫幼虫，所捕获的成幼虫进行蚊虫种类鉴定，调查出当地蚊虫种类组成及其蚊虫多样性特征。（2）对现场采集的成虫，经分类鉴定、计数后分装，置于液氮罐内低温保存，带回实验室进行研磨、接种C6/36细胞分离病毒和RT-PCR病毒鉴定。结果：（1）采用诱蚊灯共捕获5属15种12384只蚊虫，其中乙型脑炎媒介三带喙库蚊和致倦库蚊为当地优势蚊种，分别占捕获蚊虫总数的59.95%(7424/12384)和10.90%(1350/123

7、84);疟疾传播媒介中华按蚊和微小按蚊为当地优势蚊种，分别占捕获蚊虫总数的19.31%(2391/12384)和4.13%(511/12384)；该3个县6个现场调查点蚊虫物种丰富度均较低，多样性指数、均匀度、生态优势度基本相似；采用双层叠帐捕蚊法共捕获4属11种327只蚊虫，其中登革热传播媒介白纹伊蚊为优势蚊种（占捕获蚊虫总数19.88%），其次为尖斑伊蚊（18.35%）和骚扰阿蚊（14.07%）；幼虫调查法共采集到四龄幼虫6属18种238条蚊虫，其中三带喙库蚊数量较高。（2）共从116组蚊虫病毒分离中发现29组