Springer.Freitag.R.(ed.).Modern.Advances.in.Chromatography.(Springer.2002)(T)(251s)

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1、PrefacePrefaceDuetotheirversatilityandresolution,chromatographicseparationsofcomplexmixturesofbiologicalsareusedformanypurposesinacademiaandindustry.Ifanything,recentdevelopmentsinthelifescienceshaveincreasedtheinterestandneedforchromatographybeitforqualitycontrol,proteomicsorthedown-strea

2、mprocessingofthehighvalueproductsofmodernbiotechnology.How-ever,themany“challenges”ofpresentdaychromatographyandespeciallyoftheHPLCofbiomacromoleculessuchasproteins,arealsopresentinthemindofanypractitioner.Infact,someoftheselatterweresuchhindrancesthatmuchresearchwasnecessaryinordertooverc

3、omeandcircumventthem.Thisbookintroducesthereadertosomeoftherecentlyproposedsolutions.Capillaryelec-trochromatography(CEC),forexample,thelatestandmostpromisingbranchofanalyticalchromatography,isstillhinderedfromfindingbroaderapplicationbydifficultiesrelatedtosomethingassimpleasthepackingofa

4、suitablecolumn.ThelatestsolutionsforthisbutalsothestateofartofCECingeneralaredis-cussedinthechapterwrittenbyFrantisekSvec.Thedifficultyofcombiningspeed,resolutionandcapacitywhenusingtheclassicalporousbeadtypesta-tionaryphaseshasevenbeencalledthe“dilemmaofproteinchromatography”.Muchprogress

5、hasbeenmadeinthisareabytheadventofmonolithicandrelat-edcontinuousstationaryphases.Thecomplexnatureofmanyofthesamplestobeanalyzedandseparatedinbiochromatographyoftenrequirestheuseofsomehighlyspecific(“affinity”)ligands.Sincetheycanberaisedinaspecificmannertomanybioproducts,proteinligandssuc

6、hasantibodieshaveallowedsomeveryselectivesolutionsinthepast.However,theyalsoareknowntohavesomedis-advantages,includingtheimmunogenicity(toxicity)ofligandscontaminatingthefinalproducts,orthelowstabilityofsuchligands,whichpreventsrepeatedusageoftheexpensivecolumns.Thischallengemaybeovercomeb

7、y“molecularimprinting”,atechniques,whichusespurelychemicalmeanstocreatethe“affinity”interaction.Finallyweweremosthappytohavetwoauthorsfromindustryjoinustoreportontheirexperiencewithchromatographyasacontin-uouspreparativeprocess.Readersfromvariousfieldsthuswill