Microbal Genetics -Problems with solutions_2004.pdf

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1、GeneratedbyFoxitPDFCreator©FoxitSoftwarehttp://www.foxitsoftware.comForevaluationonly.Withsolutionhttp://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/problems/ProblemIndex:Chromosomes,DNAreplication,andgeneexpressionoBacterialchromosomesoCentraldogma:DNAreplication,transcription,andtranslationMut

2、ationsandmutagenesisoMutationfrequencyoTypesofmutationsReversionandsuppressionoTruereversionoIntragenicsuppressionoIntergenicsuppressionCross-feeding,complementationanalysis,anddominanceoCross-feedingoComplementationRecombinationandgeneticmappingoHomologousrecombinationoTwo-andthree-factorcros

3、sesoDeletionmappingPhageoLysisoLysogenyPlasmidsoReplicationandcopynumberoIncompatibilityGeneticexchangeoConjugationoTransduction1GeneratedbyFoxitPDFCreator©FoxitSoftwarehttp://www.foxitsoftware.comForevaluationonly.GeneralizedtransductionSpecializedtransductionoTransformationoBarrierstogenetic

4、exchange-Restriction-modificationTransposonsoTranspositionoPortableregionsofhomologyoFusionsandreportergenesGeneticregulationoAnalysisofregulatorymutantsoTranscriptionalinitiationoTranscriptionalterminationoTranslationalregulationoPost-transcriptionalregulationInvitrogeneticanalysisoDNAcloning

5、andsequencingoPhysicalmappingoPCRandsite-directedmutagenesisSomegoodsourcesofadditionalmicrobialgeneticsproblemsinclude:Freifelder,D.1983.ProblemsforMolecularBiology.JonesandBartlettPublishers,Boston.Maloy,S.,J.Cronan,andD.Freifelder.1994.MicrobialGenetics(Secondedition).JonesandBartlettPublish

6、ers,Boston.Schleif,R.1993.GeneticsandMolecularBiology(Secondedition).JohnsHopkinsUniversityPress,Baltimore.Smith-Keary,P.1989.MolecularGeneticsofEscherichiacoli.GuilfordPress,NY.Formorebackgroundonmanyofthesetopics,seetheMicrobialGeneticssupplement.2GeneratedbyFoxitPDFCreator©FoxitSoftwarehttp:

7、//www.foxitsoftware.comForevaluationonly.1.Chromosomes,DNAreplication,andgeneexpressionBacterialChromosomesFormanyyearsitwasbelievedthatallbacteriahaveasinglechromosome.However,morerecentlyanalysisofRhodobacteriumspher