川渝地区主要家蚕实用品种的ssr指纹图谱构建及遗传多样性分析

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1、西南大学硕士学位论文之间。以遗传相似系数0．6为界限，样品分为两个大类：其中秋白、限2、854B、湘辉、872A、7532、872B聚为一类；87lB、871A、秋丰、限1、夏芳、芙蓉、932聚为另一类。这个结果与品种所属品系一致。4．PCA分析结果与UPGMA聚类分析结果一致。5．根据聚类分析结果，筛选出17对核心引物s1502、S1503、s1504、s1508、S1509、S1514、S1515、S1516、S0201、S0213、S0214、S0216、S0217、S0219、s0301、s0302、s0

2、303。通过核心引物扩增的结果可将14个家蚕品种区分开，同时用这17对引物构建了家蚕DNA指纹数字图谱。关键字：家蚕；ssR标记；遗传多样性；指纹图谱Aba慨ctConstructionofSSRFinge印rintandAnalysisofGeneticDiVers时ofEconomicⅥ时etiesofMainSilkwonninSichuana11dChongqingAreaMasterofBiologicalEngine谢ngCandidate：Jin鲥ieWuSupeⅣisor：Prof．YongZhu

3、ProfTingLeiAbstractAsanimportanteconomicanimal，silkwomindi丘-erentecolo酉calconditionsovera10ngpedodoftime舀．aduallyfomledmallygeo掣．apllics缸ns．The缸

4、aditionaldassificanonmemodofsilkwo彻mainlybasedoneconomictraits锄dmorpholo酉calcharacters，buttheeconomictraitsare孕’ea

5、tlya仃．ectedbyerⅣir0IⅡnent，wbileitishardtodistiIlguishthemorpholo垂calcharactersaccllr纵dyforasmallerdiffhenceofvarieties．MicrosatelliteDNA(SSR)isanucleotidesequence，谢lichtakes1～6nucl鳅ides嬲auIlit，byt锄d锄r印eats，lcngmuptotensoreVenhundredsofnuclcotides，aIldwidelydi

6、stributedint11eg∞omeofeucaryⅨe．NowSSRhasalreadywidelyusedinconsnuctionofmoleculargeneticmap，identi丘cationofgenIlplaSmresource，a11alysisofgeneticdiVersity’genelocation，QTLmappingaIlddoIlingaIldauxiliaryselectionofm01ecularma】墩er．etc．Thesilk、Ⅳo硼gemlpl雏mresource

7、DNAfinge叩rintusingSSRmarker，itcaIlpr0VideaconVenientmemodf-ortlleselectionofV撕ehes，forbreedingsilkwo吼witIlrecessiVegeneaIldcreatingsill叫。衄Varietieswithspecialcharacters，itcanimproVetheaccuracyofani丘cialselectionprocess，shortenmeVarie够breedingcycle，andIII西南大学硕

8、士学位论文providescienti6ceVidencefortlleideIltification，g∞eticeValuationandutilizationofsilkwonn．Inthisstudy，ittakes14kindsofmaillsilkwo眦V耐etiesinSichuaIlaIldChongqingareaasmaterials，usingpolyacryl锄idegelelcc岫phoresistoa11alyzePCRproduct，a11dsilVerstaining，studie

9、Sonsilkwo蛐microsatellitep01ymorphism，themainresearchresultsal-easf’ollOws：1．26pairsofSSRprimerswithbetterpolymo印hismwereselectedf的m36pairS，asf01lows：S1502，S1503，S1504，S1507，S1508，S1509，S1