ADHD发病机制中酪氨酸羟化酶表达调控的研究

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1、温州医科大学硕士学位论文分类号：R72单位代码：10343学号：151002297硕士学位论文论文题目:ADHD发病机制中酪氨酸羟化酶表达调控的研究研究生姓名：潘景学科专业：儿科学类型：学术型指导教师：吴丽慧二O一八年五月温州医科大学硕士学位论文论文题目:ADHD发病机制中酪氨酸羟化酶表达调控的研究答辩委员会主席：吕海涛教授苏州大学附属儿童医院答辩委员会成员：褚茂平教授温州医科大学附属第二医院庄捷秋教授温州医科大学附属第二医院论文答辩日期：2018年5月20日温州医科大学硕士学位论文目录一、英文缩略注释··

2、·····································1二、中文摘要···········································2三、英文摘要···········································4四、ADHD发病机制中酪氨酸羟化酶表达调控的研究前言················································6材料与方法······································

3、····8结果···············································33分析与讨论·········································42小结···············································43参考文献···········································44附录···············································47五

4、、致谢··············································48六、综述及参考文献····································49七、独创性············································70温州医科大学硕士学位论文英文缩略注释(Abbreviation)英文简称英文全称中文全称ADHDAttention-deficithyperactivitydisorder注意力缺陷多动障碍APAmmoniumPe

5、rsulfate过硫酸铵BSABovineserumalbumin牛血清白蛋白DADopamine多巴胺DEPCDiethylpyrocarbonate焦碳酸二乙酯DMEMDulbecco'sModifiedEagleMedium细胞培养基EDTAEthyleneDiamineTetraaceticAcid乙二胺四乙酸EBEthidiumbromide溴化乙锭TEMDN,N,N',N'-Tetramethylethylenediamine四甲基乙二胺FBSFetalBovineSerum胎牛血清HSHors

6、eSerum马血清Gal-3Galectin-3半乳糖凝集素3THTyrosineHydroxylase酪氨酸羟化酶GAPDHGlyceraldehyde-3-phosphatedehydrogenase三磷酸甘油醛脱氢酶hhour小时HRPHorseradishPeroxidase辣根过氧化物酶Kbkilobases千碱基L-DOPAl-3,4-dihydroxyphenylalanine左旋多巴minminute分PBSPhosphateBufferedSaline磷酸盐缓冲液PAGEPolyacryl

7、midegelelectrophoresis聚丙烯酰胺凝胶电泳PCRPolymerasechainreaction聚合酶链式反应PFCprefrontalcortex额前皮质PVDFpolyvinylideneFluoride聚偏二氟乙烯QPCRQuantitativepolymerasechainreaction定量聚合酶链反应SHRSpontaneouslyHypertensiveRats自发性高血压大鼠WKYWistar–KyotoWistar京都大鼠LVLentivirusvector慢病毒载体WB

8、WesternBlot蛋白质印记法KDKDa千道尔顿TrisTris-hydroxymethyl-aminomethan三羟甲基氨基甲烷mRNAmessengerRNA信使核糖核酸1温州医科大学硕士学位论文ADHD发病机制中酪氨酸羟化酶表达调控的研究中文摘要目的：探讨ADHD大鼠模型SHR大鼠脑前额皮质(prefrontalcortex，PFC)中酪氨酸羟化酶(tyrosinehydroxylase,TH)在半乳