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时间:2019-03-09
《弥漫性脑损伤后脑水肿和c--fos、c--jun蛋白的变化》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、分类号:1wH卜112//118//1。1191//,11。1/15111学号:20101381掣1必心必泰山匡亏弦硕士学位论文论文题目:弥漫性脑损伤后脑水肿和c—Fos、c—Jun蛋白的变化作者姓名:马宪存学科、专业:外科学学位类型:专业学位型研究方向:神经外科指导教师:郑修启教授入学时间:2010年9月论文工作起止时问:2012年3月~2013年3月中文摘要英文摘要目录⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·1符号说明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·3前言⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯
2、⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯44刖舌⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯材料与方法⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯”6结果⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯‘11讨论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·15结论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯‘18参考文献⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯
3、⋯⋯⋯⋯⋯⋯⋯⋯19综述⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯。21致谢⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯·32原创性声明⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯33弥漫性脑损伤后脑水肿和c.Fos、c.Jun蛋白的变化IIlllllllllllllllIMIIIIllUllllUllll研究生:马宪存Y2440939专业:神经外科学导师:郑修启教授中文摘要目的研究弥漫性脑损伤后不同时间内脑水肿和c.Fos、c.Jun蛋白的变化,探
4、讨脑损伤后的生理变化为临床治疗奠定基础。方法采用Marmarou方法制作大鼠弥漫性脑损伤模型,在损伤后不同时间(1h,3h,6h,12h,ld,3d,7d),检测脑组织含水量的变化及用免疫组织化学技术和图像分析方法观察c.Fos和c—Jun的表达规律。结果伤后脑组织含水量,在1h即有所提高,ld达高峰,至7d仍高于对照组水平。c.Fos和c.Jun在伤后1h即可见到大量表达:c—Fos在伤后3h达高峰(P5、,随后有所降低,至3d又达高峰(P6、rationofC-Fosandc-JunIntervalsinthediffusebraininjury(DBI)andinvestigateitsrelationtotheinjuryintervals.MethodsBasedonMarmou’SmodelofDBIandtookthesamplesat30min,lh,3h,6h,12h,ld,3d,7dafterthebraininjury.C—Fosandc—Junweredetectedbyimmunohisto-chemicalstainingincombinationwithimagingana7、lysis.ResultsThewatercontentofinjuryedbraintissusesincreased1hourpost—injury,peaked24hourspost—injury,thendecreased?A.t1hourpost—trauma,asignificantincreaseinc—Fosandc—Junpostivecellswasobsered.Theexpressionlevelofc—Fospeakedat3h,thendecreasedlately,andthesecondpeaktimewasthe3dpost—t8、rauma.Thec—f
5、,随后有所降低,至3d又达高峰(P6、rationofC-Fosandc-JunIntervalsinthediffusebraininjury(DBI)andinvestigateitsrelationtotheinjuryintervals.MethodsBasedonMarmou’SmodelofDBIandtookthesamplesat30min,lh,3h,6h,12h,ld,3d,7dafterthebraininjury.C—Fosandc—Junweredetectedbyimmunohisto-chemicalstainingincombinationwithimagingana7、lysis.ResultsThewatercontentofinjuryedbraintissusesincreased1hourpost—injury,peaked24hourspost—injury,thendecreased?A.t1hourpost—trauma,asignificantincreaseinc—Fosandc—Junpostivecellswasobsered.Theexpressionlevelofc—Fospeakedat3h,thendecreasedlately,andthesecondpeaktimewasthe3dpost—t8、rauma.Thec—f
6、rationofC-Fosandc-JunIntervalsinthediffusebraininjury(DBI)andinvestigateitsrelationtotheinjuryintervals.MethodsBasedonMarmou’SmodelofDBIandtookthesamplesat30min,lh,3h,6h,12h,ld,3d,7dafterthebraininjury.C—Fosandc—Junweredetectedbyimmunohisto-chemicalstainingincombinationwithimagingana
7、lysis.ResultsThewatercontentofinjuryedbraintissusesincreased1hourpost—injury,peaked24hourspost—injury,thendecreased?A.t1hourpost—trauma,asignificantincreaseinc—Fosandc—Junpostivecellswasobsered.Theexpressionlevelofc—Fospeakedat3h,thendecreasedlately,andthesecondpeaktimewasthe3dpost—t
8、rauma.Thec—f
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