大鼠胃肠道不同部位cajal间质细胞与慢传输型便秘关系

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1、目录英文缩略表（Abbreviations）·································1中文摘要··················································2英文摘要（Abstract）········································4正文······················································7前言·························

2、·····························7材料与方法················································9结果·····················································13讨论·····················································21结论··················································

3、···26下一步研究方向···········································27参考文献·················································28附录·····················································33致谢·····················································34综述······················

4、·······························35参考文献·················································43英文缩略词表（Abbreviations）英文缩写英文全称中文名称STCslowtransitconstipation慢传输型便秘ICCinterstitialcellofcajalCajal间质细胞PBSphosphatebufferedsolution磷酸盐缓冲液H&Ehematoxylinandeosin苏木素和伊红

5、ENSentericnervoussystem肠神经系统minminute分钟ggram克RNAribonucleicacid核糖核酸cGMPcyclicguanosinemonophosphate环磷酸鸟苷mgmiligram毫克SCFstemcellgrowthfactor干细胞生长因子1大鼠胃肠道不同部位Cajal间质细胞与慢传输型便秘的关系中文摘要目的：观察慢传输型便秘（slowtransitconstipation，STC）模型大鼠胃肠道Cajal间质细胞（interstitialce

6、llofcajal，ICC）的分布特点与含量改变，全面评估ICC在STC发病机制中的作用。对象和方法：选取24只健康Wistar大鼠，6-8周龄，体质量（120±10）g，随机分为正常对照组（thecontrolgroup）和STC模型组（themodelgroup）（n=12）。模型组给予含复方苯乙哌啶混悬液灌胃，对照组给予同等剂量的生理盐水灌胃，每5d记录1次大鼠大便粒数、大便干质量及大鼠体质量。饲养90d后停药1周，测定胃肠道传输时间，大鼠解剖后随机选取胃窦、小肠、结肠组织，进行苏木精-伊

7、红染色，以观察STC大鼠胃肠道组织的病理组织学变化。并对ICC的特异性标志物c-kit细胞进行免疫组化染色，观察ICC在STC大鼠胃肠道不同部位的分布情况及数量变化。采用SPSS16.0统计软件包进行统计分析，计量资料结果以x±s表示，计数资料以例数(百分比)来表示。两组间差异显著性检验采用t检验,等级资料采用秩和检验。以P<0.05提示差异有统计学意义。结果：实验动物全部进入结果分析（无死亡记录）。（1）STC模型组日均粪便粒数21.17±7.00粒/d少于对照组34.43±4.61粒/d，平

8、均每粒粪便质量175.10±27.70mg/粒大于对照组143.03±24.89mg/粒，差异有统计学意义（P<0.01）;（2）模型组首粒黑便排出时间为475.43±62.37min，长于对照组的350.5±103.84min，差异有统计学意义（P<0.05）。（3）造模第5d两组间的平均体质量差异无统计学意义（P>0.05），而造模第90d两组间的平均体质量模型组为346.08±25.25g，明显低于对照组415.57±18.31g，存在明显统计学差异（P<0.01）。（4）两组大鼠胃窦、小