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1、M™ETHODSINMOLECULARBIOLOGYSeriesEditorJohnM.WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForothertitlespublishedinthisseries,gotowww.springer.com/series/7651M™ETHODSINMOLECULARBIOLOGYToll-LikeReceptorsMethodsandProtocolsEditedbyClaireE.McCoyandLukeA
2、.J.O’NeillSchoolofBiochemistryandImmunology,TrinityCollegeDublin,Dublin2,IrelandEditorsClaireE.McCoyLukeA.J.O’NeillSchoolofBiochemistryandImmunologySchoolofBiochemistryandImmunologyTrinityCollegeDublin,TrinityCollegeDublin,Dublin2,IrelandDublin2,IrelandISBN:978-1-934115-72-5e-ISBN:978-1-
3、59745-541-1ISSN:1064-3745e-ISSN:1940-6029DOI:10.1007/978-1-59745-541-1LibraryofCongressControlNumber:2008943238©HumanaPress,apartofSpringerScience+BusinessMedia,LLC2009Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress
4、,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereaft
5、erdevelopedisforbidden.Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.Whiletheadviceandinformationinthisbookarebelievedtobetrueandaccuratea
6、tthedateofgoingtopress,neithertheauthorsnortheeditorsnorthepublishercanacceptanylegalresponsibilityforanyerrorsoromissionsthatmaybemade.Thepublishermakesnowarranty,expressorimplied,withrespecttothematerialcontainedherein.Printedonacid-freepaperspringer.comPrefaceThediscoveryofToll-likere
7、ceptors(TLRs)initiatedarenaissanceforthefieldofinnateimmunity,leadingtothediscoveryofmultiplereceptorsandsignallingpathwaysimportantforhostdefence.TLRsareexpressedhighlyoncellsoftheimmunesystemandplayacru-cialroleininitiatinganeffectiveimmuneresponsethatprotectsthehostaga
