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1、ActaPhysiologicaSinica667Dec.1999,51(6),667～674EFFECTOFINTRACAROTIDADMINISTRATIONOFADENOSINEONTHEACTIVITYOFAREAPOSTREMANEURONSINBARODENERVATEDRATSCHENS3HUANG,HERUI2RONG(DepartmentofPhysiology,HebeiMedicalUniversity,Shijiazhuang050017)ABSTRACTToobservetheeffectofintracarotidadministrationof
2、adenosineontheelectricalactivityofareapostrema(AP)neurons,76spontaneousactiveunitswererecordedfrom45sino2aorticdenervatedSprague2Dawleyratsusingextracellularrecordingtechnique.Theresultsobtainedareasfollows.(1)Follow2ingintracarotidadministrationofadenosine(Ado,25μg/kg),thedischargerateof2
3、9outof42unitsde2creasedmarkedlyfrom6126±0175to4174±0176spikes/s(P<0101),whereasthatof6unitsincreasedfrom4113±0177to4172±0183spikes/s(P<0105),andtheother7showednoresponse.Bloodpressure(BP)andheartrate(HR)wereunalteredthroughouttheexperiment.(2)82phenyltheophylline(82PT,15μg/kg),anonselectiv
4、eadenosinereceptorantagonist,completelyblockedtheinhibitoryeffectofAdoin10u2nits.(3)SelectiveA1adenosinereceptorantagonist,82cyclopentyl21,32dipropylxanthine(DPCPX,50μg/kg),blockedtheeffectofAdoin12unitstoaremarkableextent.(4)Glibenclamide(500μg/kg),ablockerofATP2sensitivepotassiumchannel,
5、abolishedtheeffectofAdoin12units.TheaboveresultsindicatethatAdocaninhibitspontaneouselectricalactivityofAPneurons,whichismediatedbyadenosineA12receptorwiththeinvolvementofATP2sensitivepotassiumchannels.Keywords:adenosine;areapostrema;spontaneoussingle2unitdischarge;82phenyltheophylline;82c
6、yclopentyl21,32dipropylxanthine;glibenclamide[1]Adenosine(Ado)mayplayaroleasacentralmodulatorincardiovascularregulation.OurpreviousstudydemonstratedthatAdodoesresultinanenhancedneuronalexpressionofFos(amarkerofneuronalactivation)inbaroreflexpathway,includingthenucleusofthesolitarytract(NTS
7、),area[2]postrema(AP)androstralventrolateralmedulla(RVLM),specificallyintheAP.SucharesultsuggeststhatAdoisabletoaffecttheactivityofsomeregionsinCNSinvolvedinthebaroreflexfunc2tion,especiallyinAP.Furthermore,wehaveshowedthatAdoexertsaninhibitoryactiononthespon2
