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ID:34564880
大小:827.44 KB
页数:68页
时间:2019-03-08
《Pharmaceutical Impurities- A Mini-Review.pdf》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、HPLCTroubleshootingGuideClickonsectionsbelowtogodirectlytothatsectionoftheTroubleshootingGuideHPLCTroubleshootingUweD.Neue,WatersCorporation1.ColumnLife-Time27.OptimalFlowRates2.VariableRetentionTimes28.CarbonLoad3.DriftingRetentionTimes29.pHControl4.Column-to-ColumnandBatch-to-B
2、atch30.MobilePhaseCompositionReproducibility31.ColumnContamination5.SamplePreparationProblems32.MethodVerification6.SourcesofPeakTailing33.DoublePeaksinSugarSeparations7.Normal-PhaseChromatography34.MethodControl8.SystemVolume,DeadVolume,DwellVolume35.MobilePhasepH9.TransferofGra
3、dientMethods36.Signal-to-NoiseImprovements10.CloggedSystem37.Overload11.ColumnPlate-Count38.ColumnDurability12.ColumnBackpressure39.FastAnalysisandColumnBackpressure13.PeakAreaFluctuations40.ColumnBackflushing14.GhostPeaks41.SelectivityShift15.DependenceofRetentionTimesonpH42.New
4、Method16.ColumnEquilibration43.NegativePeaks17.ColumnConditioning44.Tricky,Tedious,TimeConsuming18.ComplexSampleMatrices45.FastSeparations19.HydrophobicCollapse46.BuffersforLC/MS20.BaselineNoise47.AlkalineBuffersforRPLC21.Narrow-boreColumns48.Post-ColumnDerivatization22.SampleSol
5、vent49.GradientDwellVolume23.GradientScaling50.BufferCapacity24.ColumnStorage51.FlowRateChangesandQuantitation25.Paired-IonChromatography52.Analysisofpolarcompounds26.HydrolyticStabilityofReversed-PhasePackings121.ColumnLife-Timecartridgewithasimilarchemistryastheseparationcolumn
6、workswellforthisproblem.Q.:Mycolumnlastedonlyforabout100injections.AfterAnotherandmorepowerfulapproachistouseaguardthattime,thepeaksbecamedistortedandtheplate-countscolumn.Theprecolumnservesasasacrificialcolumntopwereverylow.What’swrong?thatisreplacedwhentheproblemoccurs.Forbestp
7、erformance,youshoulduseaguardcolumnthatcontainsA.:100injectionsisindeedashortlife-time.Undernormalexactlythesamepackingastheanalyticalcolumnandiscircumstances,onecanexpectacolumntobeinservicepackedwiththesamehighperformancepackingtechniqueasmuchlonger.Inordertodeterminewhatiswron
8、g,weneedtheanalyticalcolumn.Ifyouuseprec
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