DNA Analysis Notebook .pdf

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1、Great!PromegahasToDoList:everything1.PurifygenomicDNAIneed!2.Amplifysequence3.Clean-upreaction4.Cloneamplimer5.Pickupcoffeefor8ammeeting!DNAAnalysisNotebookTableofContentsGenomicDNAPurification2PCRClean-Up28OverviewOverviewWizard®GenomicDNAPurificationKitWizard®SVGelandPCRClean-UpSystemWiz

2、ard®SVandSV96GenomicDNAWizard®SV96PCRClean-UpSystemPurificationSystemsWizard®MagneSil®PCRClean-UpSystemMagneSil®BloodGenomic,MaxYieldSystemWizard®PCRPrepsDNAPurificationSystemMagneSil®ONE,FixedYieldBloodGenomicSystemOrderingInformationMagneSil®Genomic,FixedTissueSystemMagneSil®KF,GenomicSy

3、stemWizard®Magnetic96DNAPlantSystemCloningPCRDNA34OrderingInformationOverviewBasicSubcloningAmplifyingDNA13DirectMammalianExpressionDirectBacterialExpressionOverviewPCRCloningTechniquesOptimizationofPCROrderingInformationRoutinePCRProofreadingPolymerasesDNAAnalysisTools43HotStartMethodolog

4、ydNTPsBenchtopDNAMarkersOrderingInformationLaddersandDigestMarkerswww.promega.com•techserv@promega.comGenomicDNAPurificationPromegahasavarietyofsolutionsforyourgenomicLookforthesesymbolstofindthesystemrightDNA(gDNA)purificationneeds.Promegahasforyourapplication.automatedmethodsforblood,cul

5、turedcells,mammaliantissueandplantpurifications.AllsystemsBloodproducehigh-qualityDNAreadyforamplification.Mostautomatedsystemsusemagneticparticle-basedCulturedCellstechnologies.TheWizard®SVtechnologiesintroducemembrane-basedpurificationformanualorautomatedAnimalTissuepurifications.TheWiza

6、rdGenomicDNAPurificationKitisatrulyversatilesolution-basedsystemtomanuallyPlantTissueisolatehighmolecularweightgDNAfromavarietyofstartingmaterials.Thisgentlesolution-basedmethodBacteriaproducesgDNAsuitableforamplification,Southernblottingandgenomiccloning.YeastPleasenote,intheflowchartstha

7、tfollow,FixedTissueNEWPromegasystemsarelistedbywhichprotocolscomeintheTechnicalLiteraturesentwiththesystem.OtherapplicationstoothergDNAsourcesBuffycoatormayexist(e.g.,BloodprotocolsfortheBloodWizardSVGenomicSystem).whitebloodcells?PleasecontactPromegaTechnical