乙醇的基因毒性及原花青素的保护作用研究

《乙醇的基因毒性及原花青素的保护作用研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、沈阳药科大学博士学位论文摘要withdrawal，DNAdamageinducedbychronicethanoladministrationcouldbealmostcompletelyrepaired，suggestingthattheDNAauto-repairingabilityinthebrainisquitestrong．Afterethanoltreatment，theconcentrationsofethanolandacetaldehydeinbrainregionsofmiceweredi

2、fferentandtheconcentrationsofethanolincerebellumandhippocampuswerehigherthantheotherbrainregions．’nleresultssuggestedthatethanolinducedDNAdamageincerebellumandhippocampuswasrelativetothehighconcentrationofetllanti．Oxygen—derivedfreeradicalshavebeenimplicate

3、dinmanydiseaseprocesses，includingethanol-inducedneurotoxicity．Ethanolexposurehasbeenshowntoinducethegenerationofreactivefreeradical／nvitroandinvivo．Ourpreviousstudyshowedthatethanol-inducedDNAdamageWasresultedfromtheethanol-inducedoxidativestress．Therefore，

4、itisreasonabletoassumethatantioxidantsmighthaveprotectiveeffectsonethanol-inducedoxidativeDNAdamage．Inpresentstudy，itWasclearlydemonstratedthattheisolatedgrapeseedprocyanidinfractionsandotherantioxidantssuchasascorbicacid，vitaminEandresveratrolcouldmarkedly

5、decreasethelevelsofDNAsingle—strandbreaksinducedbyethan01．Inotherwords，thegrapeseedprocyanidinsandotherantioxidantsCallbeusedinpreventionandtreatmentoftheailmentsincentralnervoussysteminducedbyethanolabuse．Wehavedemonstratedthatcerebellumandhippocampus．espe

6、ciallycerebellum,arethemaintargetsofgenotoxicityofethan01．Furtherstudieswereconductedusingprimaryculturesofratcerebellargranularneuronstoinvestigatethegenotoxicityofethanol／nvitroandthereleasingofROS，theHO一1andOGGlmRNAexpressionweresimultaneouslymeasured．Wh

7、etherthegrapeextractsandredwineextractshaveprotectiveeffectson，ethanolinducedDNAdamagewerealsodetected．ItWasfoundthatethanolcouldinducesignificantDNAdamageandreleasingofROSinratcerebellargranularneurons．Ethanolexposedfor3hand6hcouldupregulatetheHO—landOGG1m

8、RNAexpressionsuggestingacompensatoryregulationoftheantioxidantdefenseandDNArepairsystem．Inthepresentstudy,grapeandredwineextractscouldpreventethanol—inducedDNAdamageandROSgenerationinratcerebellargranu