巴马香猪血液生理化指标参考标准地建立及enu诱变下血液指标异常个体地筛选

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1、EstablishedthereferencestandardofBamaminiaturepigs?bloodphysiologicalandbiochemicalindicatorsandscreenedabnormalindividualofbloodparametersinENU-inducedBamaminiaturepigmutantsAbstractBamaminiaturepigshaveirreplaceableadvantagesthanotheranimalsintheplaceofgenetic,development,dis

2、easemodelsandorganxenotransplantation,andarewidelyusedinthestudyofthecardiovascularsystemandheterogeneousorgantransplantation.ThehematologicandbiochemicalofBamaminiaturepigsareimportantbasicindicatorsinpathology,physiologyandtoxicologyresearchwork,andarealsoanimportantbasistodeterminetheph

3、ysiologicalstateofBamaminiaturepigs.ButthefeedingstandardsofBamaminiaturepigshavenotunitedbecausetheyareanemerginglarge-scaleexperimentalanimal,manyresearchdepartmentstakerestrictedfeedingonBamaminiaturepigstopreventfleshing,whichmayinterferethedeterminationofphysiologicalandbiochemicalind

4、icators.GeneticallymodifiedbyChemicalmutagenENUisanewmethodtoprepareanimalmodelsofdisease.Itisslsofaster,higherintensityofmininggeneandproducedahugenumberofmutantanimalsashorttimethantraditionalgeneknockoutandtrangenosis.DuetotherandomnessanduncertaintyofENUmutagenesis,techniquesandmethods

5、ofscreeningmutantanimalmodelsofdiseaseareparticularlyimportant.Itisnotonlywellimproveandsupplementthescreeningmethodsofanimalmodelsofdisease,butalsocanobtainindividualofabnormalbloodparametersthroughthescreeningonthebloodphysiologicalandbiochemicalindexesofF1generationofBamaminiaturepigs.I

6、nourstudy,336weaningBamaminiaturepigswererandomlydividedintocontrolgroup,80%ofthedietgroupand50%ofthedietgroupaccordingtothedifferentrestrictedfeedinglevels,bloodsampleswerecollectedfromprecavaat60days?ageand90days?age,sevenimportanthematologicparametersandtenphysiologicalparametersweredet

7、ermined,thenwecountedthedifferencesofindicatorsunderdifferentrestrictedfeedinglevels,wegottheunderlyingdataofBamaminiaturepigs?bloodphysiologicalandbiochemicalindexes,andestablishareferencerangeofphysiologicalandbiochemicalindicators.ThensevenF0generationboars