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1、1MANUSCRIPTTITLE:2FunctionalPropertiesandExpressionQuantitativeTraitLociforPhosphate13TransporterGmPT1inSoybean4RUNNINGTITLE:FunctionandeQTLMappingofGmPT11a2a1115HAINASONG,ZHITONGYIN,MAONICHAO,LIHUANING,DANZHANG&DEYUE16YU*17NationalCenterforSoybeanImprovement,NationalKeyLaboratoryofCro
2、pGeneticsand8GermplasmEnhancement,NanjingAgriculturalUniversity,Nanjing210095,China.29ArticleJiangsuProvincialKeyLaboratoryofCropGeneticsandPhysiology,YangzhouUniversity,10Yangzhou225009,China.11*Correspondingauthor.Tel:+86-25-84396410;Fax:+86-25-84396410;E-mail:dyyu@njau.edu.cna12Thes
3、eauthorscontributedequallytothearticle.131415Dateofsubmission:15Jul201316Numberoftables:217Numberoffigures:518Supplementarydata:119Wordcount:5095ThisarticlehasbeenacceptedforpublicationandundergonefullpeerreviewbuthasnotbeenthroughtheAcceptedcopyediting,typesetting,paginationandproofre
4、adingprocess,whichmayleadtodifferencesbetweenthisversionandtheVersionofRecord.Pleasecitethisarticleasdoi:10.1111/pce.12170Thisarticleisprotectedbycopyright.Allrightsreserved.20ABSTRACT21Phosphate(Pi)remobilizationwithinaplantiscriticalforplantsurvivalunderPi-limitingconditions.22Inthis
5、paper,asoybeanphosphatetransportergene,GmPT1,wascharacterized.Amarkedinduction23ofGmPT1transcriptwasobservedinyoungleaves,matureleavesandlateralrootsduringlong-term24Pistarvation.TransgenictobaccoplantscontainingtheGmPT1genewereobtainedusingan25Agrobacterium-mediatedtransformationsyste
6、m.Comparedwithwild-typeplants,transgenicplants26showedsignificantincreasesinphosphorus-useefficiency(PUE),photosystemIIfunction,totaldry27weightandseedweightunderPi-deficientconditions.GmPT1expressionlevelsandPUEwere28Articledeterminedinasoybeanrecombinantinbredlinepopulationduringapot
7、experimentthatwas29conductedtomeasurechlorophyllfluorescenceparameters,photosyntheticrate(PN)andseedyield.30CorrelationanalysisrevealedthatGmPT1expressionlevelshadsignificantlypositivecorrelations31withseedyield,PUE,PNandthequantumyieldofPSIIprimaryphotochemistry(ΦPSII).Expression32q