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时间:2019-03-06
《凡纳滨对虾4个选育群体遗传多样性的ssr分析》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、第29卷第4期广东海洋大学学报Vol.29No.42009年8月JournalofGuangdongOceanUniversityAug.2009doi:10.3969/j.issn.1673-9159.2009.04.002凡纳滨对虾4个选育群体遗传多样性的SSR分析1211谢丽,陈国良,叶富良,栗志民(1.广东海洋大学水产学院,广东湛江524025;2.湛江海茂水产生物科技有限公司,广东湛江524000)摘要:采用9对微卫星引物分析了凡纳滨对虾4个选育群体(Molokai、OI、SIS和KonaBay)的遗传多样性。结果表明:9对微卫星引物共检测到32个等位
2、基因,每个位点等位基因数2~7个,平均3.5556个,平均有效等位基因数2.4727;各位点的观测杂合度(Ho)0.0260~0.6234,期望杂合度(He)0.2631~0.7855;各群体平均观测杂合度从小到大依次为SIS(0.3833)3、缘关系最远。关键词:凡纳滨对虾;选育群体;遗传多样性;微卫星中图分类号:S917文献标志码:A文章编号:1673-9159(2009)04-0005-05GeneticDiversityofFourSelectedStocksofLitopenaeusVannameiasRevealedbySSRMarker1211XIELi,CHENGuo-ling,YEFu-liang,LIZhi-min(1.FisheriesCollege,GuangdongOceanUniversity,Zhanjiang524025,China;2.ZhanjiangHaimaoAq4、uaticBiologyTechnologyCo.,Ltd,Zhanjiang524000,China)Abstract:Geneticdiversityof4selectedstocks(Molokai,OI,SISandKonaBay)ofLitopenaeusvannamewasassessedby9microsatellitemarkers.Theresultsshowedthatthenumberoftotalalleleswere32.Thenumbersofalleleofeverylocuswasrangedfrom2to7.Themeanall5、elenumber(Na)was3.5556,Heterozygosityvalue(Ho)observedwas3.5556.Observedheterozygosityvalue(Ho)was0.0260to0.6234andexpectedheterozygosityvalue(He)was0.2631to0.7855.TheorderofmeanHovalueswasSIS(0.3833),KonaBay(0.4052),Molokai(0.4278)andOI(0.4444).Thecoefficientofgenedifferentiationbet6、weenthestocks(Gst)was0.1583,thereweremajorgeneticdifferentiationamongthese4selectedpopulations.Basedontheresultsofgeneticsimilarity,thegeneticdistanceandUPGMAdendrogramwasconstructed.AndwefoundthattherelationshipofpopulationofOIandKonaBaywastheclosest,andMolokaiandKonaBaypopilationwe7、refollowed.SISandMolokaipopulationhavethemostdistantrelationship.Theresultsmayprovideatheoreticalbasisforconservation,exploitationandbreedingofLitopenaeusvannamei.Keywords:Litopenaeusvannamei;selectedstocks;geneticdiversity;microsatelite[3]凡纳滨对虾(Litopenaeusvannamei),俗称南美丢失、品质下降、生长缓慢。8、为保护和充分利用优白对虾
3、缘关系最远。关键词:凡纳滨对虾;选育群体;遗传多样性;微卫星中图分类号:S917文献标志码:A文章编号:1673-9159(2009)04-0005-05GeneticDiversityofFourSelectedStocksofLitopenaeusVannameiasRevealedbySSRMarker1211XIELi,CHENGuo-ling,YEFu-liang,LIZhi-min(1.FisheriesCollege,GuangdongOceanUniversity,Zhanjiang524025,China;2.ZhanjiangHaimaoAq
4、uaticBiologyTechnologyCo.,Ltd,Zhanjiang524000,China)Abstract:Geneticdiversityof4selectedstocks(Molokai,OI,SISandKonaBay)ofLitopenaeusvannamewasassessedby9microsatellitemarkers.Theresultsshowedthatthenumberoftotalalleleswere32.Thenumbersofalleleofeverylocuswasrangedfrom2to7.Themeanall
5、elenumber(Na)was3.5556,Heterozygosityvalue(Ho)observedwas3.5556.Observedheterozygosityvalue(Ho)was0.0260to0.6234andexpectedheterozygosityvalue(He)was0.2631to0.7855.TheorderofmeanHovalueswasSIS(0.3833),KonaBay(0.4052),Molokai(0.4278)andOI(0.4444).Thecoefficientofgenedifferentiationbet
6、weenthestocks(Gst)was0.1583,thereweremajorgeneticdifferentiationamongthese4selectedpopulations.Basedontheresultsofgeneticsimilarity,thegeneticdistanceandUPGMAdendrogramwasconstructed.AndwefoundthattherelationshipofpopulationofOIandKonaBaywastheclosest,andMolokaiandKonaBaypopilationwe
7、refollowed.SISandMolokaipopulationhavethemostdistantrelationship.Theresultsmayprovideatheoreticalbasisforconservation,exploitationandbreedingofLitopenaeusvannamei.Keywords:Litopenaeusvannamei;selectedstocks;geneticdiversity;microsatelite[3]凡纳滨对虾(Litopenaeusvannamei),俗称南美丢失、品质下降、生长缓慢。
8、为保护和充分利用优白对虾
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