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1、作物学报ACTAAGRONOMICASINICA2011,37(9):1559−1568http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2011.01559水稻抗纹枯病导入系的构建及抗病位点的初步定位11,**11311高晓清谢学文许美容王磊石英尧高用明朱苓华1,*1,2周永力黎志康12中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重
2、大科学工程,北京100081;InternationalRiceResearchInsti-3tute,DAPOBox7777,MetroManila,Philippines;安徽农业大学,安徽合肥230036摘要:以我国目前生产中广泛应用的杂交稻恢复系蜀恢527和明恢86为轮回亲本,以江西丝苗为供体亲本配制BC2F2混合群体。通过逐代人工接种筛选,获得49个BC2F4抗病导入系。对抗病选择导入系进行基因型分析,利用基于遗传搭车原理的卡方检验对等位基因导入频率的偏离进行检测,共检测到12个显著位点,2个群体定位的
3、QTL各有1个在以往不同群体中也能被检测到。主要农艺性状分析表明,非接种条件下,选择导入系群体的抽穗期、株高和千粒重等与轮回亲本无显著差异;在接种的条件下,在2个试验地点中,分别有6个和2个蜀恢527和明恢86背景的株系抗病性显著高于轮回亲本,产量与轮回亲本无显著差异。上述结果为水稻抗纹枯病分子育种提供了有用的信息和中间材料。关键词:水稻;回交导入系;纹枯病;QTLDevelopmentofIntrogressionLinesandIdentificationofQTLsforResistancetoSheath
4、Blight11,**1131GAOXiao-Qing,XIEXue-Wen,XUMei-Rong,WANGLei,SHIYing-Yao,GAOYong-Ming,ZHU11,*1,2Ling-Hua,ZHOUYong-Li,andLIZhi-Kang1InstituteofCropSciences/NationalKeyFacilityforCropGeneResourcesandGeneticImprovement,ChineseAcademyofAgriculturalSciences,23Beijing
5、100081,China;InternationalRiceResearchInstitute,DAPOBox7777,MetroManila,Philippines;AnhuiAgriculturalUniversity,Hefei230036,ChinaAbstract:Inthisstudy,tworandomBC2F2populationsweredevelopedbycrossandbackcross,inwhichtwomajorhybridre-storerlinesShuhui527andMing
6、hui86wereusedasrecurrentparentsrespectively,andJiangxisimiaousedasdonor.ThebulksoftheBC2F2populationswereinoculatedwithRhizoctoniasolanitoscreenthelinesresistanttosheathblight,and49resistantintro-gressionlines(ILs)wereselected.ThegenotypesofILswereanalyzedwit
7、hevenlydistributedpolymorphicSSRmarkers,anda2totalof12significantlociwereidentifiedbyχtestanalysis.Amongthem,twoallelicfrequency-deviationlociwerelocatedinthesameornearregionsidentifiedinthepreviousstudies.Inthefieldtestwithoutartificialinoculation,therewasno
8、significantdif-ferencebetweenalltheagronomictraitsinvestigatedintheselectedILsandtheirrecurrentparents.Underheavilydiseasedcondi-tions,sixILswithShuhui527backgroundandtwoILswithMinghui86b
