bt转基因棉氮代谢生理变化的研究

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1、第25卷第4期扬州大学学报(农业与生命科学版)Vol.25No.42004年12月JournalofYangzhouUniversity(AgriculturalandLifeSciencesEdition)Dec.2004Bt转基因棉氮代谢生理变化的研究1222陈后庆,刘　燕,张　祥,陈德华(1.扬州大学实验农牧场,江苏扬州,225009;2.扬州大学江苏省作物栽培生理重点开放实验室,江苏扬州,225009)摘　要:在田间条件下,对Bt杂交棉科棉1号和常规棉新洋822及其亲本的氮代谢生理情况进行研究。结果表明:Bt棉的氮代谢较强,以盛铃期最为明显,表现为叶片全氮含量明显升高,

2、科棉1号和新洋822在盛铃期分别比亲本增加36101%和18.96%,而棉铃全氮含量显著下降。氨基酸和可溶性蛋白质含量显著提高,盛铃期科棉1号和新洋822叶片游离氨基酸分别增加10.86%和9.44%,可溶性蛋白分别增加9.82%和8.31%。叶片硝酸还原酶(NR)、丙酮酸转氨酶(GPT)活性增强,科棉1号和新洋822盛蕾期NR活性比其亲本分别高28.37%和12.83%;GPT活性在盛蕾期和盛铃期比亲本分别增加10.51%、4.38%和39.04%、29.05%,蛋白酶活性则明显下降。因此对于Bt基因导入的棉花品种在推广应用过程中应及时调控棉株氮代谢生理强度,以保持营养生长和

3、生殖生长的平衡。关键词:Bt转基因棉;氮代谢;生理变化中图分类号:S562文献标识码:A文章编号:16714652(2004)04002005StudyonthePhysiologicalChangeofNitrogenMetabolisminBtTransgenicCotton222CHENHou-qing,LIUYan,ZHANGXiang,CHENDe-hua(1.ExperFarm,YangzhouUniv,Yangzhou,225009,China;2.KeyLabofCropCultiandPhysioofJiangsuProvince,YangzhouUniv,Y

4、angzhou,225009,China)ABSTRACT:TwoBttransgeniccottoncultivars,oneishybridforKemianNo.1,anotherisconventionalforXingyang822,wereusedtostudythechangeofnitrogenmetabolismcomparingtheirparentsunderfieldconditionduringthe2001,2002cottongrowingseasons.Theresultsshowedthattheintensityofnitrogenmetab

5、olismwashigherforBtcottonthanthatfortheirparents,especiallyinpeakbollperiod.Incomparisontotheirparents,thetotalnitrogencontentintheleafin2creased,theapparentincreasewas36.01%inbollperiodforKemianNo1,18.96%forXingyang822.Thetotalnitrogeninbolldecreasedsignificantly.TheNPandGPTactivitywashighe

6、r,theNPactivityincreased28.37%forKemianNo1and12.83%forXingyang822duringpeaksquareperiod;theGPTactivityincreased10.51%and4138%duringpeaksquareperiodandpeakbollstageforKemianNo.1,39.04%and29.05%forXingyang822.Thecontentoffreeaminoacidandsolubleproteinincreasedsignificantly,duringbollperiod,the

7、freeaminoacidincreased10186%forKemianNo.1,9.44%forXingyang822,thesolubleproteinrose9.82%forKemianNo.1,8.31%forXingyang822.theproteaseactivi2tydeclinedsignificantly.Theseresultsindicatedthatthemediationofnitrogenmetabolismwasnecessaryforkeepingtheba