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1、LETTERdoi:10.1038/nature12364Geneticprogramsinhumanandmouseearlyembryosrevealedbysingle-cellRNAsequencing12233111ZhigangXue*,KevinHuang*,ChaochaoCai,LingboCai,Chun-yanJiang,YunFeng,ZhenshanLiu,QiaoZeng,11322LimingCheng,YiE.Sun,Jia-yinLiu,SteveHorvath&GuopingFanMammali
2、anpre-implantationdevelopmentisacomplexprocessIntriguingly,clusteranalysesshowedthatbothpronuclearand1–4involvingdramaticchangesinthetranscriptionalarchitecture.zygote(1-cellstage)embryosclusteredcloselytogetherbutawayfromWereporthereacomprehensiveanalysisoftranscript
3、omedynamicsoocytesandcleavageembryos,indicatingthatthe1-cellstageexhibitsafromoocytetomorulainbothhumanandmouseembryos,usingdistincttranscriptomepattern.Comparisonsbetweenmatureoocytessingle-cellRNAsequencing.Basedonsingle-nucleotidevariantsinand1-cellembryosidentifie
4、d149differentiallyexpressedgenes(falsehumanblastomeremessengerRNAsandpaternal-specificsingle-discoveryrate(FDR),5%,.2-foldchange),79and70ofwhichwerenucleotidepolymorphisms,weidentifynovelstage-specificmono-up-anddownregulatedin1-cellembryos,respectively(Fig.1c).Inter-
5、allelicexpressionpatternsforasignificantportionofpolymorphicestingly,approximatelyhalfoftheupregulatedgenesremainhighlygenetranscripts(25to53%).Byweightedgeneco-expressionnetworkexpressedin2-and4-cellstagesbutaredownregulatedatthe8-cellanalysis5,6,wefindthateachdevelo
6、pmentalstagecanbedelineatedstage,whereastheotherhalfisfurtherupregulatedatthe8-cellstageconciselybyasmallnumberoffunctionalmodulesofco-expressed(Fig.1d).Byperformingsimilarsingle-cellRNA-seqinmousepre-genes.Thisresultindicatesasequentialorderoftranscriptionalchangesim
7、plantationembryos(SupplementaryTable1),weobserved520trans-inpathwaysofcellcycle,generegulation,translationandmetabo-criptsthatwereupregulatedinpronucleicomparedtomatureoocyteslism,actinginastep-wisefashionfromcleavagetomorula.Cross-(SupplementaryFig.1),confirmingthatb
8、othhumanandmousespeciesspeciescomparisonswithmousepre-implantationembryosrevealexhibitaconservedminorwaveofZGAbeforethemajor
