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1、中国人兽共患病学报2009,25(6)ChineseJournalofZoonoses573文章编号:1002-2694(2009)06-0573-053液相中炭疽杆菌形貌的原子力显微镜观测1,2222212张丽萍,平芮巾,杨鹏飞,米超,王静,岳启安,胡孔新摘要:目的利用原子力显微镜液相模式观测炭疽杆菌形态,获得液相中炭疽杆菌芽胞和繁殖体对于溶菌酶的形态学抗性变化数据。方法应用原子力显微镜液相模式观察炭疽杆菌繁殖体及芽胞的超微结构,并对其主要指标进行测量比较。结果1×PBS溶液中的炭疽杆菌繁殖体,菌体杆状,竹节样排列,菌体间连接致密,长度为2,757.30±1227.08
2、6nm,宽度为1,710.90±226.10nm,Rq为15.447±3.418nm,Ra为13.239±2.733nm;炭疽杆菌芽胞椭圆形,多散在分布,表面平滑,长度为2,014.00±227.155nm,宽度为1,264.10±132.180nm,Rq为22.803±5.660nm,Ra为18.010±4.568nm。0.01mg/mL溶菌酶溶液中的炭疽杆菌繁殖体形态不规则,表面凹凸起伏,边缘粗糙,有囊状突起,长度为2,836.00±1025.137nm,宽度为2,449.00±212.78nm,Rq为17.068±4.427nm,Ra为14.776±3.746nm;
3、0.01mg/mL溶菌酶溶液中的炭疽杆菌芽胞,形态规则,边缘平滑,未见凹凸起伏和囊状突起,而1mg/mL溶菌酶溶液环境中的炭疽杆菌芽胞形态不规则,表面起伏,变化较大,其长度为2,155.00±202.663nm,宽度为1,344.00±162.631nm,Rq为24.849±3.427nm,Ra为20.869±2.550nm。结论通过原子力显微镜液相模式下的观察和测量,清楚地显示了溶液中炭疽杆菌的微观形貌及其变化,0.01mg/mL的溶菌酶就能使炭疽杆菌繁殖体发生形态学变化,而炭疽杆菌芽胞形貌发生变化则需要1mg/mL的高浓度溶菌酶的作用。关键词:原子力显微镜;炭疽杆菌;
4、形态学;液相模式;溶菌酶中图分类号:R378.8文献标识码:AMorphologicalobservationonBacillusanthracisbyusingatomicforcemicroscopyinfluidtappingmodeZHANGLi2ping,PINGRui2jin,YANGPeng2fei,MIChao,WANGJing,YUEQi2an,HuKong2xin(DepartmentofMicrobiology,WeifangMedicalCollege,Shandong261042,China)ABSTRACT:Theatomicforcemicr
5、oscopyinliquidtappingmodewasusedtoexaminetheultrastrureturesofvegetativeformsandsporesofBacillusanthracisinliquidconditionandtoobtainthedataontheirchangeofmorphologicalresistancetolysozyme.WefoundthatthemicrostructuresofB.anthracisvegetativeformandsporewereimagedinaseriesofdilutedlyso2zym
6、esolutionsinordertoobservethemorphologicchangesofbacteria.B.anthracisdisplayeddifferentstructuresin1×PBSsolution,0.01mg/mLlysozymesolutionand1mg/mLlysozymesolution.Thelength,width,RqandRaofthevegetativeformofB.anthraciswere2757.30±1227.086nm,1710.90±226.10nm,15.447±3.418nmand13.239±2.733n
7、mre2spectively;whilethoseoftheoval,dispersedandsmoothsporesofB.anthraciswere2014.00±227.155nm.1264.10±132-180nm,22.803±5.660nmand18.010±4.568nmrespectively.Themorphologyofvegetativeformsin0.01mg/mLoflyso2zymesolutionappearedtobeirregularonthesurfaceandroughinthesurr
