增殖性糖尿病视网膜病变患者玻璃体腺苷浓度和增殖膜中cd73表达研究

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1、增殖性糖尿病视网膜病变患者玻璃体腺苷浓度和增殖膜中CD73表达研究郎敏1袁容娣2陈春林1霍妍1邹欢1叶剑1(400042,重庆，第三军医大学大坪医院野战外科研究所眼科1)（400037，重庆，第三军医大学新桥医院眼科2）[摘要]目的通过检测增殖性糖尿病视网膜病变(proliferativediabeticretinopathy，PDR)患者玻璃体腺苷浓度及增殖膜CD73的表达，探讨增殖性糖尿病视网膜病变与玻璃体腺苷浓度及增殖膜CD73表达之间的关系。方法收集在本院眼科行玻璃体切除手术的患者共30例，分为增殖性糖尿病视网膜病变(PDR共16例16

2、眼）组和对照组（孔源性视网膜脱离、黄斑前膜、特发性黄斑裂孔共14例14眼）。于患者玻璃体切除手术中收集玻璃体、视网膜增殖膜及黄斑前膜。应用高效液相荧光检测法检测患者玻璃体腺苷的浓度，Westernblot检测膜样本中CD73蛋白的表达。结果人眼玻璃体腺苷浓度及增殖膜CD73酶在PDR组表达高于对照组（62.37±11.47μMvs30.34±6.41μM；0.84±0.08vs0.39±0.05），差异均具有统计学意义(t=9.480,P=0.000；t=7.15,P=0.002)。结论腺苷和CD73在PDR患者的玻璃体及增殖膜中的表达较非视网

3、膜新生血管性疾病患者明显升高，提示二者可能参与了增殖性糖尿病视网膜病变新生血管的形成。[关键词]腺苷；CD73；增殖性糖尿病视网膜病变；视网膜新生血管[中图法分类号]R774.1[文献标志码]AExpressionandsignificanceofadenosineandCD73inproliferativediabeticretinopathyLangMin，ChenChunling,Huoyan，ZouHuan,YeJian（DepartmentofOphthalmology，DapingHospital，theThirdMilitaryM

4、edicalUniversity，Chongqing400042，China）;YuanRongdi.DepartmentofOphthalmology，XinqiaoHospital，theThirdMilitaryMedicalUniversity，Chongqing400037，China.[Abstract]ObjectiveToinvestigatethelevelsofadenosineinvitreosandtheexpressionofCD73infibrovascμlarmembraneofpatientswithprolif

5、erativediabetic[基金项目]第三军医大学校青年创新人才基金(2010XQN37)[通信作者]叶剑，E-mail:yejian1979@163.comretinopathy(PDR).MethodsWeselected30patientswhounderwentvitrectomiesintheOphthalmologyatDapingHospitalanddividedthemintotwogroups.Sixteenpatients(16eyes)wereingroupdiseasesofretinalneovascμlariz

6、ation(PDRgroup).Fourteenpatients(14eyes)wereincontrolGroup,whichwaswithoutneovascμlarizationinretinal.Inthisgroup,rhegmatogenousretinaldetachment,idiopathicmacμlarholeandmacμlarepiretinalmembranewerecontained.ThelevelsofadenosineinvitreousweremeasuredbyHPLC.TheexpressionofCD

7、73inpreretinalmembraneswasmeasuredbywesternblot.ResμltsThelevelsofadenosineingroupPDRwashigherthanthatingroupcontrol(t=9.480,P=0.000).TheexpressionofCD73ingroupPDRwashigherthanthatingroupcontrol(t=7.15,P=0.002).ConclusionTheexpressionofandenosineandCD73mayinvolvedintheprogre

8、ssofneovascμlarizationwithproliferativediabeticretinopathy.[Keywords]Adenos