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1、局部氧疗对高温复合伤大鼠创面肌肉组织影响的实验研究910?解放军护理杂志20040ct;21(10)局部氧疗对高温复合伤大鼠创而肌肉组织影响的实验研究李亚洁,杨瑞,罗炳德(1•第一军医大学南方医院护理部;2.第一军医大学南方医院高温医学教研室,广东广州510515)摘要:目的探讨局部氧疗法对高温复合创伤大鼠创面肌肉组织的影响,为湿热环境的创伤伤口护理提供实验依据•方法建立24只人鼠腹部创伤模型,随机将其分为常温对照组,常温氧疗组,高温对照组和高温氧疗组4组,每组6只,让其分别接受常温暴露,常温氧疗,高温暴露和高温氧疗,然后进行光镜和透射电镜观察.结果高温对照组创而肌肉的组织形态
2、和超微结构变化最为剧烈,肌纤维明显变性萎缩甚至变性坏死成碎片,毛细血管扩张充血明显,大量炎性细胞入侵组织,组织间隙水肿严重;大量的线粒体发生不可逆的空泡样变和髓样变,糖原颗粒剧减,巨噬细胞退变;高温氧疗组明显好于高温对照组,肌纤维仅肿胀而未发生萎缩变性,线粒体也未发生空泡样变,糖原颗粒与高温对照相比较多,可见少量未退变的巨噬细胞.结论高温复合创伤应激比单因素的创伤应激显着,高温高湿环境创伤组织肌纤维损伤严重,局部氧疗法可降低高温高湿环境因素和创伤因素的损伤作用,从而延缓创面局部组织进一步的病理变化.关键词:局部氧疗法;高温;创伤;肌肉;大鼠中图分类号:R459.6文献标识码:A
3、文章编号1008—9993(2004)10—0010—05ExperimentalStudyontheEffectofTopicalOxygenTherapyonMusclesoflRatsSustainingCom-poundTraumainHyperthermicEnvironmentLIYa-jie,YANGRui,LUOBing-de(DepartmentofNursing,NanfangHospital,FirstMilitaryMedicalUniversity,Guangzhou,5105159China)Abstract:Objrecfivelbinvestig
4、atetheinfluenceoftopicaloxygentherapyonmuscularwoundsofratsinhotandhumidenvironment.MethodsTraumaticmodelswereestablishedin24rats,whichwererandom.lydividedintofourgroups3ncludingnormalenvironmentcomparisongroup(NECgroup,n:6),normalenvironmentoxygentherapygroup(NEOgroup,n=6),hotandhumidenviro
5、nmentcomparisongroup(HHEgroup,n=6),andhotandhumidenvironmentoxygentherapygroup(HHOgroup,n:6).Thenallratsweresampledtoobservechangesofmuscleunderanopticalmicroscopeandelectronmicroscope.ResultsPathohistologyofmusclecellswasobviouslychangedinHHEgroup.whereswollenmitochon-driawithcristaelossand
6、vacuolization.derangedmyofibrillaewithsomelossofmyofilaments,reducedglycogengranules,anddegeneratedmacrophageswereseen.DamagetomusclefibersinHHOgroupwasnotSOseriousasthatinHHEgroup.DamagetomuscIecellsinNECgroupwasslighterthanthatinHHEgroup.TheultrastructureofmusclecellsinNEOgroupwasnormal.Co
7、nclusionlnhotandhumidenvi—ronment,pathohistologicchangesofwoundsweremoreserious,andtopicaloxygentherapycouldattenu-ateultrastructuralchangesofmuscletissues.Keywords:topicaloxygentherapy;hightemperature;trauma;muscle;rats高温复合创伤是热区部队常遇到的应激因素,在湿热和创伤双重