pdms微芯片通道表面修饰方法及其在氨基酸分离-分析中的应用-研究

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1、ABSTRACTRecently,microchipcapillaryelectrophoresissystemisbecominganewlyrapidlydevelopedresearchtechnologyandhasanextensiveapplicationperspective．Meanwhile，ithaswonanincreasingattentionandprovidedapowerfulplatformforanalyticalchemistryduetoitsreducedtimeforanalyticalprocess，highsamplethroughput，

2、lowconsumptionofsamplesandreagents，easyintegrationandautomaticallycontr01．Polydimethylsiloxane(PDMS)hasbecomeapopularmaterialforbuildingmultifunctionalmicrofluidicdevicesmainlyduetoitsgoodopticaltransparency，easysealingwithothermaterials，nontoxicity，lowcost，easyfabrication，increasingversatility,

3、relativelylowcuringtemperatureandgoodbiocompatibility．However．PDMSmicrofiuidicdeviceemployedformicrochipcapillaryelectrophoresisalsoshowssomedefectswhichhinderitsapplicationscopeinlifescience．Thesedefe：ctsincludethelowdensityofchargeonthesurfaceofPDMSmicrochip，theunstableelectroosmoticflow(EOF)，

4、extremehydrophobicityofPDMSmicrochip．easyadsorptionofanalytesontothemicrochipchannelsurface，etc．Toovercometheabovedefects，surfacemodificationtechniqueshavebeendevelopedtoimprovethesurfacepropertiesofPDMSinthiswork，whichwereasfollows．1．Afacile．environmentallyfriendlyandeconomicalmethodbasedoninsi

5、tuchemicallyinducedsynthesisstrategywasdevelopedfor’themodificationofaPDMSmicrochipchannelsurfacewithpolydopamine／goldnanoparticles(PDA／AuNPs)tocreateahydrophilicandbiofoulingresistantsurface．Here，dopamine(DA)asreductantandmonomer,andHAuCl4asoxidanttotriggerDAself-polymerizationandthesourceofAuN

6、Ps．werefilledintoPDMSmicrochanneltoimmobilizewell．distributedandrobustPDA／AuNPscoatingontothesurfaceofPDMSmicrochipchannelbytheinsituchemicallyinducedstrategy．ComparedwiththenativePDMSmicrochipchannel，themodifiedoneexhibitedhighstabilityandsuppressedEOF，muchbetterwettability,andlessnonspecificad

7、sorptiontowardsfiveaminoacids．Fastandefficientseparationoffiveaminoacidssuchasarginine(Arg)，proline(Pro)，histidine(His)，valine(Val)andthreonine(Thr)suggestedgreatlyimprovedelectrophoreticperformanceonthePDA／AuNPs—coatedPDMSm