pdms微芯片通道表面修饰方法及其在氨基酸分离分析中的应用研究

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1、ABSTRACTRecently,microchipcapillaryelectrophoresissystemisbecominganewlyrapidlydevelopedresearchtechnologyandhasanextensiveapplicationperspective．Meanwhile，ithaswonanincreasingattentionandprovidedapowerfulplatformforanalyticalchemistryduetoitsreducedtimeforanaly

2、ticalprocess，highsamplethroughput，lowconsumptionofsamplesandreagents，easyintegrationandautomaticallycontr01．Polydimethylsiloxane(PDMS)hasbecomeapopularmaterialforbuildingmultifunctionalmicrofluidicdevicesmainlyduetoitsgoodopticaltransparency，easysealingwithother

3、materials，nontoxicity，lowcost，easyfabrication，increasingversatility,relativelylowcuringtemperatureandgoodbiocompatibility．However．PDMSmicrofiuidicdeviceemployedformicrochipcapillaryelectrophoresisalsoshowssomedefectswhichhinderitsapplicationscopeinlifescience．Th

4、esedefe：ctsincludethelowdensityofchargeonthesurfaceofPDMSmicrochip，theunstableelectroosmoticflow(EOF)，extremehydrophobicityofPDMSmicrochip．easyadsorptionofanalytesontothemicrochipchannelsurface，etc．Toovercometheabovedefects，surfacemodificationtechniqueshavebeend

5、evelopedtoimprovethesurfacepropertiesofPDMSinthiswork，whichwereasfollows．1．Afacile．environmentallyfriendlyandeconomicalmethodbasedoninsituchemicallyinducedsynthesisstrategywasdevelopedfor’themodificationofaPDMSmicrochipchannelsurfacewithpolydopamine／goldnanopart

6、icles(PDA／AuNPs)tocreateahydrophilicandbiofoulingresistantsurface．Here，dopamine(DA)asreductantandmonomer,andHAuCl4asoxidanttotriggerDAself-polymerizationandthesourceofAuNPs．werefilledintoPDMSmicrochanneltoimmobilizewell．distributedandrobustPDA／AuNPscoatingontoth

7、esurfaceofPDMSmicrochipchannelbytheinsituchemicallyinducedstrategy．ComparedwiththenativePDMSmicrochipchannel，themodifiedoneexhibitedhighstabilityandsuppressedEOF，muchbetterwettability,andlessnonspecificadsorptiontowardsfiveaminoacids．Fastandefficientseparationof

8、fiveaminoacidssuchasarginine(Arg)，proline(Pro)，histidine(His)，valine(Val)andthreonine(Thr)suggestedgreatlyimprovedelectrophoreticperformanceonthePDA／AuNPs—coatedPDMSm