类风湿性关节炎小鼠模型的制备与刺五加皂苷e对其治疗作用的分析

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1、类风湿性关节炎小鼠模型的制备及刺五加皂苷E对其治疗作用的研究英文摘要EleutherosideEAmelioratesArthritisSeverityinCollagen-InducedArthritisMiceModelbySuppressingInflammatoryCytokineReleaseAbstractObjectives:AneffectiveandpracticalmicemodelofrheumatoidarthritiswasestablishedbyinjectingofbovinecollagentypeII.Thechan

2、gesofbodyweight,thegeneralscoreofjoint,jointradiologicalscore,anti-IIcollagenantibodylevelandthelevelofinflammatorycytokinesinserumwereusedtoevaluatedtheseverityofarthritis.ThetherapeuticeffectsofdifferentdosesofEleutherosideEontherheumatoidarthritismicemodelweresubsequentlytest

3、ified.Finally,thepotentialmechanismofEleutherosideEwasinvestigated.Methods:①SPFmaleDBA/1micewerefeeduntil7-8weeks,weighting18-25g.ThebovinecollagentypeIIandcompleteFreund’sadjuvantemulsionwasusedtoinducerheumatoidarthritismodelbysubcutaneousinjectioninthebackasaprimaryimmunizati

4、on.Afterthreeweeks,thebovinecollagentypeIIandincompleteFreund’sadjuvantemulsionwasinjectedsubcutaneouslyatthebaseofthetailasaboostimmunization.②ThemodelmicewererandomlydividedintofivegroupsincludingTripterygiumgroup(15mg/kgorally),low-doseEEgroup(15mg/kgorally),medium-doseEEgrou

5、p(30mg/kgorally)andhigh-doseEEgroup(60mg/kgorally),andthemodelgroupwasinjectedwithvehicle(orally)alone.③Thesignsandsymptomsofarthritis(includingweight,jointswelling),radiographicchangesandhistopathologicalchangesindifferenttreatmentgroupswereobservedandrecorded.TheseraofCIAmiceu

6、sedinthisexperimentwerecollectedanddetectedtheconcentrationofcytokinesandtheiranti-IIcollagenantibodies.④THP-1cellswereco-culturedwithdifferentdosesofEEfor48h.ThesupernatantswerecollectedanddetectedthelevelofTNF-αandIL-6.Results:①Therheumatoidarthritismodelwasdevelopedsuccessful

7、lybyinjectingbovinecollagentypeII.②Comparedtovehicle-treatedCIAmice,30mg/kgand60mg/kgIII英文摘要类风湿性关节炎小鼠模型的制备及刺五加皂苷E对其治疗作用的研究EEtreatmentobviouslydecreasedthearthritisscoresandbodyweightlossinCIAmice.ThehindpawthicknessinCIAmicewassignificantlydecreased(P<0.01).Noticeably,thearthrit

8、isscores,bodyweightlossandthehindpawthicknessof