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1、MONOSPORECROSSBREEDINGANDSCREENINGOFNEWSTRAINSRESISTANTTODISEASEOFOYSTERMUSHROOMABSTRACTInthisexperiment,eightparentstrainsofoystermushroomincludingPleurotusostreatus,P.pulmonarius,P.citrinopileatusandP.diamorwereusedforcollectingbasidiospores,throughthemonosporecrossbreeding,thehybridprog
2、enystrainswereobtained,andtheywereexaminedthecompetitiveabilityagainstTrichodermaspp.andPseudomonastolaasiiusingadualculturesystemandbaggrowingsystem.Thepurposeofthisstudyisforbreedingandscreeningnewstrainsresistancetodiseaseofoystermushroom.Theresultsareasfollows:1.309monocaryoticstrainso
3、foystermushroomwereobtained,mostofthemwereselectedforcrossbreeding,and864crosscombinationswereproduced.Then77newcrossingprogeniesstrainswereobtainedbybearingexperiments.Throughsecondscreening,27strainswereobtainedwithhighergrowthrate,vigorouslymyceliumgrowth,perfectshapeoffruitbadyanddiffe
4、rentwithparents.2.ThroughRAPDanalysisbetweenhybridstrainsandparents,25hybridstrains,whicharethenewstrainshavesignificantgeneticdifferenceswithparents,2hybridstrainsgeneticvariationofsterilelineswasclosetoparents.3.Bycomparingthemyceliumgrowthwithdifferenttemperature,fourstrainsasP2,P3,P6an
5、dP8aregrowingfasterat25℃.ThestrainsasP6,P19,P20aregrowingfasterat30℃,andthestrainasP19isgrowingfastestinallstrains,thegrowthrateofmyceliawasfasterthantheirparentsas4.8mm/d,8.9mm/d.SixstrainsasP2,P20,P23,P24,P25hadtheabilityofhightemperaturetolerance.4.Comparedexperimentsamongmaterials,frui
6、tbodyyieldsoftenstrainswerehigherthantheirparentalstrains,fivestrainswerehigherthanoneoftheirparentalstrains.ThehighestyieldasP23andP30.III5.ThoughexaminedthecompetiveabilityofhybridstrainsagainstTrichodermaharzianumT12andTrichodermapleurotumT33,Theantagonisticexperimentshowedthatthreestra
7、insasP4,P7,P14hadstrongerresistanttotheT12,fivestrainsasP10,P14,P16,P20,P21hadstrongerresistanttoT33.Infruitingexperiment,thefruiting,among1parentwere100%,the7strainswerelower.6.Throughtheflatantagonism,the27hybridstrainsandparentalstrainsinaccordancewiththele
