生物材料微图形生物活性表面对细胞生长行为的调控-研究

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1、重庆大学硕士学位论文茎塞塑鐾ABSTRACTThestudyofbonedefectanditsrepairationisstillagreatprobleminorthopaedics。thedesiredbiomaterialsinorthopaedicsshouldinthenatureofosteoconductionandosteoinductivity，surfacemodificationisaimportantapproachtocreatebiocompatibilityinthesurfaceofbiomaterials．T

2、hemainpurposeofthispaperIStofabricatedifferentshapeandsizebioactivemoleculemicropatternsonthesurfaceofbiomaterialstoregulatethegrowthandthefunctionofosteoblast，andcreateasurfacewhichhavegoodbiocompatibility。Forthispurpose，wecreatedifferenttypesotbioactivemicropatternsthrough

3、microcontactprintinginbiomaterials(titaniumandPolystyrene)，andstudytheeffectsofmicropatternsintheregulationofosteoblastadhesion．spreadandproteinexpression．Themaincomponentandresult1Sas1；bllows：1．Wemodifythesurfaceoftitaniumandobtainaaldehydefunctionlizationsurface，thenusealb

4、uminfrombovineserum(BSA)andhumanfibronectin(FN)asmodelprotein，tocreatedifferentsizeandshapeofmicropattemsinthealdehydemodifiedtitaniumsurfacethroughmicrocontactprinting．TheanalysisofXPS、contactan91eandAFMshowweSUCCESSineachstepsofmodification，thenweobserveBSAmicropatternsand

5、FNmicropattemsinthesurfaceoftitaniumthroughConfocalLaserScanningMicroscope(CLSM)．Weusethesamemethedtoanalysetheproteinmicropatternsinthepolyst)，rrene(PS)．andtakecompareofmicropatternsintwodi慌rentmaterials，wefindthestabilityofthemicropattemcovalentbondingintitaniumishighertha

6、nthemicropatternphysicaladsorpinPS，buttheaccuracyofthePSsampleishigherthanthetitaniumsample；Intheanalysisofdifferentsizesofmicropatterns，wefindbothDositivestampandnegativestampweredeformedwhenthevalueofawas1essthanorequalto5pm，whichresultedinreplicationerrors，andthepatternsf

7、abricatedbythenegativestampwerebetterthanthoseofpreparedbythepositivestamp．2．Newbomratosteoblastcellsareseededontheseproteinmicropattern．functionalizedPSandusemicroscopetoobservetheeffectionofthemicropatteminosteoblastcelladhesion，spreadingandproliferation；Asthesametimewedye

8、thecytoskeleton，nucleolus，collagenIandosteocalcinthroughinununofluorescence