生物材料微图形生物活性表面对细胞生长行为的调控研究

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1、重庆大学硕士学位论文茎塞塑鐾ABSTRACTThestudyofbonedefectanditsrepairationisstillagreatprobleminorthopaedics。thedesiredbiomaterialsinorthopaedicsshouldinthenatureofosteoconductionandosteoinductivity，surfacemodificationisaimportantapproachtocreatebiocompatibilityinthesurfaceofbiomaterials．Themainpurposeo

2、fthispaperIStofabricatedifferentshapeandsizebioactivemoleculemicropatternsonthesurfaceofbiomaterialstoregulatethegrowthandthefunctionofosteoblast，andcreateasurfacewhichhavegoodbiocompatibility。Forthispurpose，wecreatedifferenttypesotbioactivemicropatternsthroughmicrocontactprintinginbiomat

3、erials(titaniumandPolystyrene)，andstudytheeffectsofmicropatternsintheregulationofosteoblastadhesion．spreadandproteinexpression．Themaincomponentandresult1Sas1；bllows：1．Wemodifythesurfaceoftitaniumandobtainaaldehydefunctionlizationsurface，thenusealbuminfrombovineserum(BSA)andhumanfibronecti

4、n(FN)asmodelprotein，tocreatedifferentsizeandshapeofmicropattemsinthealdehydemodifiedtitaniumsurfacethroughmicrocontactprinting．TheanalysisofXPS、contactan91eandAFMshowweSUCCESSineachstepsofmodification，thenweobserveBSAmicropatternsandFNmicropattemsinthesurfaceoftitaniumthroughConfocalLaser

5、ScanningMicroscope(CLSM)．Weusethesamemethedtoanalysetheproteinmicropatternsinthepolyst)，rrene(PS)．andtakecompareofmicropatternsintwodi慌rentmaterials，wefindthestabilityofthemicropattemcovalentbondingintitaniumishigherthanthemicropatternphysicaladsorpinPS，buttheaccuracyofthePSsampleishigher

6、thanthetitaniumsample；Intheanalysisofdifferentsizesofmicropatterns，wefindbothDositivestampandnegativestampweredeformedwhenthevalueofawas1essthanorequalto5pm，whichresultedinreplicationerrors，andthepatternsfabricatedbythenegativestampwerebetterthanthoseofpreparedbythepositivestamp．2．Newbomr

7、atosteoblastcellsareseededontheseproteinmicropattern．functionalizedPSandusemicroscopetoobservetheeffectionofthemicropatteminosteoblastcelladhesion，spreadingandproliferation；Asthesametimewedyethecytoskeleton，nucleolus，collagenIandosteocalcinthroughinununofluorescence