毛冬青甲素对脑缺血后notch通路的调控作用与神经元再生

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1、HES1的表达具有促进作用，提示脑缺血再灌注后早期IA促进Notch信号通路的激活。关键词：毛冬青甲素，局灶性脑缺血再灌注，Notch信号通路，神经干细胞，神经元-3-万方数据TheregulationofIlexoninAonNotchsignalingpathwayandneurogenesisafterCerebralIschemia-ReperfusioninRatsABSTRACTObjective:ToinvestigatetherelationshipbetweentheeffectofIlexoninAonnotchsignali

2、ngpathwayandproliferationanddifferentiationofneuralstemcellsaftercerebralischemia-reperfusion,andexplorethepossiblemechanismofrepairafterbraininjury.Methods：ThemodelofMiddlecerebralarteryocclusion(MCAO)wasestablishedtocerebralischemia-reperfusionmodelbyplacinganintraluminalfi

3、lamentattheoriginofleftmiddlecerebralarterytwohours.Theratswereassignedrandomlyinto:control,shamoperation,modelandIlexoninAgroups.ModelgroupandIAgroupwerereperfuseddifferernttimerespectively.NeuronogicaldeficitswereassessedpostMCAObyLonga’sscoringmethod.TTCstainingwasappliedt

4、ovarifyexperimentalanimalmodelsandprovidepositioningmark.ThenumberofBrdu/Nestinpositivecells,Brdu/NeuNpositivecellsandNICDpositivecellsintheperienchymaofbrainischemictissuewereobservedbyImmunohistochemistry,NICDproteinexpressionweredetectedbywesternblottingandtheexpressionofN

5、otch1,Psen1,Hes1weredetectedbyRT-PCR.Results:(1)Recoverofneurologicalfuction:NeurologicaldeficitsofmodelgroupthandIAgroupweresignificantrepairedat1dayafterischemiaandreperfusion.TheNeurologicaldeficitswererepairedprogressivelywiththeprolongationoftime.Comparewithmodelgroup,th

6、eneurologicaldeficitscoreoftheIAgroupwasmuchththless.Asignificantdifferenceoftheneurologicaldeficitscoreat3day,7dayandth14day(p<0.01,p<0.05).(2)Neurogenesisintheperienchymaofbrainischemictissue:comparewithmodelgrouptheIAgroup’sBrdu/Nestinpositivecellsincreasedththat3day(p<0.0

7、5),andreacheditspeakat7day(p<0.05)afterischemiaandthreperfusion.Brdu/Neunpositivecellsslightlyexpressat14dayinmodelgroupandIAgroup.(3)Acitvationofthenotchsignalingpathwayandexpressionofitsgenes:comparetocontrolandshamgroups,modelandIAgroupsNICDpositivecellsand’’ththproteininc

8、reased,futhermoreIAgroupsweremorethanmodelgroupsat1day,3day-4-万方数据th