ros与pi3k2fakt信号转导通路在苯致白血病中的作用

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1、中山人学硕七学位论文ROS与P13ⅪAkt信号转导通路在苯致闩血病f

2、1的作用westembIotaLssay．6．Ce¨cycleanalysisHL-60Cellswereincubatedwith3¨mol／LofBQ／PBS，C』气T+3pmol／LofBQ／PBS，IjY294002+3¨mol／LofBQ／PBSorPBSaloneduring2h．Afkrtreatment，cellswere佗一seededinafreshtissuecultureflaSk、Vithmediacontaining3％heat

3、—inactiVated凫talboVinesemmandharvestedl8hlater．ARerbeingtreatedwithPI，thecellcycleofthosesampleswasanalyzedinaFACSsortflowcytometer．7．StatisticalanalysisAllexperimentswereperfornledatleaStth．eetimes．DataareexpressedaSMean士SD．Thesignificanceofinter-groupdi腩rencewase

4、VaIuatedbyone—wayanalysisofVariance(ANOVA)followedbycomparisontestsorChi—squaretest．Significancewasacceptedatp

5、apeofthesesamplesisregular；ARerbeingincubatedwithmiddingconcemtrationofBQ(10肛mol／L)，thesh印eofsomeoftheseHL一60cellsisi丌egular．；HighconcentrationofBQ(≥l0pmo儿)couldleadceIlstodie．ForproliferationaSsays，cellswerestajned、vithalamarbluedyeandcounted．LowconcentrationofBQ(

6、≤5pmol／L)didnotsignificantlyreducecellviability邪compared、Vithcontrolcells，andhi曲concentrationofBQ(≥l0肛mo儿)could他ducecellviabilitysignificantly嬲comparedwithcontrolcells．2．Choosetheconcent门tioⅡofBQasthef0Ilowing阳seaKhdoseForproliferationaSsays，cellswerestainedwithal锄

7、arbluedye彻dcounted．3¨mo儿BQ-讹at甜HL-60cellsshowedincreaSedgro叭hoVer岫treatedcon仃ol，Wechoseit嬲thedoseinfollo、啊ngresearCh．3．Theef蕾ectofadditionofcatalaseorLY294002justbef．oreBQadditionoⅡthe—VIII—巾山人学硕上学1_《7=论文ROS与P13UAkt信号转导通路在苯致一血病I{1的作用prolieratioⅡofce¨CAr+BQ-treatedH

8、L一60cellsandLY294002+BQ．treatedHL一60cellsallshoweddecreaSedgro、VthaLscomparedwithBQ-treatedcells．4．TheefI．ectofadditionofcatalaseorLY-294002justbeforeBQadditionontheRoSgenerationincelIsROSproductionwasincreasedinBQ-treatedcelIsaScomparedwithcontroIcells；Asexpected，

9、theadditionofcatalaSejustbeforeBQadditionruducedROSgenemtiontobasalIeVels；Thereisnodi仃emCebet、veenROSproductioninBQ．treatedcellsandLY294002+BQ．tr