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1、杭州电子科技大学硕士学位论文ABSTRACTModernbiochemistrytechnologyisbasedonthecellularlevelandmolecularlevel,wherebiochemicalanalysisistowardhigh-throughput,highsensitivity,low-doseconsumptionanddynamicsanalysiswithmultiplemarkersofdevelopment,whichpresentedagreatchall
2、engetotheopticaldetectiontechniquesandinstruments.Atpresent,opticaldetectiontechnologywithhighsensitivityandhigh-speed,especiallythecurrentmicroplate-basedopticaldetectioninstrument,constitutesoneofthekeytechnologiesofmodernbiochemistryanalyzers.Forbioc
3、hemicalreactions,notonlycolorimetricanalysis,butalsochemiluminescenceandfluorescenceanalysisisnecessary;enzymaticassayandtheimmunoassaywilloccurinthesameinstrument.Inthepaper,thethreekindsofopticaldetectionmodeweredesignedformultifunctionalmicroplateana
4、lyzer,includingabsorptionspectroscopy,fluorescenceandchemical/biologicalluminescence.ThroughtheembeddedWinCEsystem,friendlyanalyzersoftwarewasdeveloped,withembeddedSQLitedatabaseformanagementofexperimentaldata.Themainfunctionofsoftwareincludesacquisitio
5、n,analysis,displayandmanagementofbiochemicalexperimentsdata.Firstly,theprincipleanddesignmethodsofabsorptionspectra,fluorescence,andchemical/biologicalluminescencedetectionforthreedetectionmodule,wasintroduced,whichisbasisoffunctionrequirementandspecifi
6、cationofmulti-functionmicroplateanalyzer.Secondly,hereintroducedtheoveralldesign,hardwaredesignandsoftwaredesign;Finally,debuggingandtestingmethodwasestablished.Theoveralldesignofthesysteminclude:WinCE-basedhuman-machineinterface,STM32-basedcontrolcircu
7、ittothemechanicalandelectricaldevicesandtheserialcommunicationprotocolofModbus-RTUbetweenSTM32andWinCE.Heretheselectionsofmasterchip,barcodescanner,steppermotor,operatingsystem,werediscussed.Herecommunicationprotocolsbetweencentralcontrolmoduleandperiph
8、eraldevicesweredesigned.Thehardwaredesignincludes:1,thedrivecircuitofmultipleanalysisopticalchannelonthreemodesofspectralabsorption,fluorescence,chemical/bioluminescence;2,thedrivecircuitofmicroplateelectromechanicalstructureofth
