illumina高通量测序技术观察剖宫产儿肠道微生物群的演替

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1、万方数据中文摘要1IlluminaMiseq测序平台能够测序肠道中所有微生物DNA序列，全面分析肠道微生物落的多样性和丰度，满足对肠道微生物群落的全面认识。2对剖宫产儿肠道微生物群进行了全面分析：(1)在门水平上，共检测出14个菌门，98％．99％以上主要为5个菌门，分别为厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)及CandidatedivisionTM7。(2)在属水平上共测得183个菌属，主要有埃希氏菌属、双歧杆菌属、克雷伯氏菌属、梭

2、状芽胞杆菌属、韦荣球菌属、乳球菌属、芽孢杆菌属、假单胞菌属等。3动态随访观察了剖宫产儿生后肠道微生物群的初始定植、分布和演替3．1门水平上，剖宫产儿生后6个月内优势菌门为厚壁菌门和变形菌门，丰度较高，其次还有放线菌门和拟杆菌门。出生后随时间推移，厚壁菌门和放线菌门是增加的；变形菌门和拟杆菌门是降低的。3．2属水平上，剖宫产儿出生后第1天粪便样本已有大量细菌定植，主要为乳球菌属、芽孢杆菌属及假单胞菌属等需氧菌和兼性厌氧菌，其大量生长繁殖消耗消化道内大量氧气，降低了肠道内酸碱度和氧化还原电位，为后期厌氧菌的定植打下基础，也表现出后期需氧菌和兼性厌氧菌定植

3、的急剧减少，专性厌氧菌如双歧杆菌属、梭状芽胞杆菌属、肠球菌属及韦荣球菌属的快速定植，表现在生后第1个月时双歧杆菌属明显增加，至6个月龄添加辅食后肠道菌群的多样性和丰度增加，双歧杆菌属成为肠道优势菌。关键词：高通量测序，肠道微生物群，足月儿，剖宫产，母乳喂养万方数据英文摘要，弋。‘一●n—UharacterizethesuccessionofgutmicrobiotaofcaesareaninfantswithhighthroughputsequencingtechnologyABSTRACTObjective：Thehumangutharborsdi

4、versemicrobesthatplayafundamentalroleinthewell-beingoftheirhost．Humansandmicrobescoexistinamultifacetedsymbioticrelationshipthatconfersbenefitstoboththehostandthemicrobe．Thegutmicrobiotaaidsinthedigestionoffoodsandacquisitionandabsorptionofnutrientsfromotherwiseindigestiblefood

5、s．Inaddition，themicrobiotaprovidesresistancetocolonizationandpreventstheovergrowthofharmfulpathogenicorganisms．Thegutmicrobiotaisalsoinstrumentalinpromotingthedevelopmentofthegutimmunesystem．Infantperiodwasacriticalstageoftheestablishmentofintestinalflorasystem，andmodeofdeliver

6、ywasakeyfactortoinfluencetheinitialcolonizationofgutmicrobiota，thepatternsofwhichcouldeffecttheriskofsubsequentallergicdiseasesandobesitybydisturbingthedevelopmentofgastrointestinalphysiologyandthematurityoftheimmunesystem．Itisobviouslydefectivetoinvestigatecomplexintestinalmic

7、roflorawimtraditionalmolecularbiologicaltechnology，suchasrealtime-PCRanddenaturinggradientgelelectrophoresis．High-throughputsequencingtechnologycoulddetectalmostalltheflorainthefeces．ThereforeweapplyIlluminahigh-throughputsequencingtechnologytoclearlyidentifycaesareaninfantinte

8、stinalfloratomorecomprehensivelyunderstandtheintestina