实验性斜视猫模型眼外肌ngf和myf5表达变化研究

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1、实验性斜视猫模型tqt曼)b11／tNGF和Myf5表达变化研究摘要目的观察共同性外斜视猫模型内直肌神经生长因子(Nervegrowthfactor，NGF)和生肌调节冈子(myocytedifferentiatjOnfactor5，Myf5)表达变化及其与斜视时问的相关性，探讨共同性外斜视病因、发病机制。方法选择处于视觉发育敏感期内幼猫(4-6w)18只，随机分为实验组和对照组，根据斜视时间实验组分为A组(斜视1个月)、B组(斜视2个月)、C组(斜视3个月)，每组各3只，对照组随机分为D、E、F组，每组各3只。通过外科手术的方法制作外斜视模型，分别于术后1个月、2个月、3个

2、月取实验组及相应对照组的内直肌，固定、脱水、石蜡包埋、切片，用免疫组织化学染色法检测内直肌NGF和Myf5平均光密度值，统计分析光密度值变化及其与不同斜视时问的相关性。结果①实验组内直肌中NGF的平均光密度值低，斜视2月组、3月组与对照组内直肌中NGF的表达量进行比较，差异县有统计学意义(P

3、NGF和Myf5表达量的降低可能参与了斜视的发生。③实验性斜视猫模型眼外肌中NGF和Myf5的表达量随斜视病程的延长而降低，该变化可能与斜视病情的进展相关。硕士研究生：崔爱芝(眼科学)指导教师：刘桂香教授关键词：外斜视；猫；眼外肌；NGF；Myf5ExpressionchangesofNGFandMyf5inextraocularmusclesofcatmodelwithexotropiaAbstractObjectiveThegoalofthisstudywastoobservetheexpressionchangesofNervegrowthfactor(NGF)andm

4、yocytedifferentiationfactor5(Myf5)inmedialrectusmuscleof'catmodelwithstrabismus．MethodWeselected18littlecats，whichwereinsensitiveperiod(4-6w)，throughsurgicalmethodstoproductexotropiamodel．Allanimalswererandomlydividedintoexperimentalandcontrolgroups．Collectedmedialrectusmusclesoftheexperime

5、ntalgroupsandcontrolgroups，respectively,afterstrabismussurgeryamomh，2months，3months．Thesecollectionwerepreparedforhistologicexmninationbyfixation，paraffinembeddingandslice．ByImmunohistochemistrystainingtodetectNGFandMyf5expressionandbyopticaldensityanalysismethodtomeasuretheaverageopticalde

6、nsityvalue．Results①Therearesignificantdifferences(P<0．05)oftheNGFexpressioninthemedialrectusmusclebetweentheexperimentalgroupandthecontrolgroup，theaftertwomonthswiththecontrolgroup，andtheafterthreemonthsgroupwiththecontrolgroup．@TheexpressionofMyf5inthemedialrectusmusclebetweentheexperiment

7、algroupandthecontrolgroup，betweentheaftertwomonthswiththecontrolgroup，andtheafterthreemonthsgroupwiththecontrolgrouparesignificantdifferences(P<0．05)．@TherearenegativerelationshipbetweenNGFandMyf5expressionandStrabismuscourseofthedisease．Conclusion①Thesu