人乳腺珠蛋白抗体鉴定及其临床意义初步的研究

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1、目录中英文词汇对照表.........................................1中文摘要.................................................2Abstract.................................................31前言...................................................52材料与方法.............................................82.1实验材料........

2、.................................82.1.1标本来源....................................82.1.2主要实验试剂...............................102.1.3部分试剂配制...............................112.1.4实验主要仪器...............................132.2试验方法........................................142.2.1抗hMAM单克隆抗体的纯化.......

3、.............142.2.2抗hMAM单克隆抗体表位鉴定..................152.2.3乳腺癌组织中hMAM含量的WesternBlot鉴定...202.2.3.1新鲜组织蛋白提取.........................202.2.3.2WesternBlot.............................202.2.4hMAM免疫组化检测..........................213结果..................................................223.1单克隆抗

4、体纯化...................................223.2抗原表位测定....................................223.3组织中乳腺珠蛋白WesternBlot鉴定................233.4免疫组化检测.....................................233.4.1不同乳腺病变中四种抗体的表达...............243.4.2四种抗体在乳腺癌组织中的表达...............253.4.2.1ab81611结果.....................

5、........253.4.2.2MDA822结果..............................273.4.2.3MEF521结果..............................293.4.2.4多抗92044结果...........................313.4.3四种抗体在乳腺癌转移组织中的表达情况.......334讨论..................................................375结论...............................................

6、...426本研究的创新点........................................427参考文献..............................................438.附录..................................................479.致谢..................................................48综述及参考文献..........................................49中英文词汇对照表英文缩写英文全

7、称中文全称hMAMhumanmammaglobin人乳腺珠蛋白PBSphosphate磷酸盐缓冲液DABdiaminobenzidine二氨基联苯胺rpmrevolutionpermin每分钟转速PCRpolymerasechainreaction聚合酶链反应Tristrishydroxymethylaminomethane三羟甲基氨基甲烷HRPhorseradishperoxidase辣根过氧化物酶SDSsodiumdodecylsulphate十二烷基磺酸钠PAGEpolyacrylamidegelelectrophoresis聚丙烯酰胺电泳SPSSStat