基于微流控芯片的环境污染物快速免疫检测技术

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1、万方数据复旦大学硕士学位论文度很高，进一步验证了该技术的可行性和稳定性。因此，该微流控免疫芯片检测技术被认为是一种适合现场快速检测草甘膦的一种新技术。该微流控免疫芯片检测技术在其他农药成分残留检测方面同样有着潜在的应用前景。关键词微流控免疫分折可替宁草甘瞵环境污染物万方数据复旦大学硕士学位论文RapidImmuneDetectionPollutantsBasedTechnologyforEnvironmentalonMicrofluidicChipsABS’I：AcTMicrofluidicsisthenewtechniquedevelopedinthepa

2、sttwodecadesthatcallprecisecontrolandmanipulatefuidsinmicronsize，typicallyinmicroliter,nanoliterorevenpicolitervolumes．Chemicalandbiologicalreactionswhicharetraditionallycarriedoutinlaboratoriesnowcanbeminiaturizedonthemicrofluidicchip．Ithasadvantagessuchashighthroughput,highreacti

3、onefficiency,enhancedmobilityaswellasreducedsampleandreagentconsumption．Microfluidicshasbeenutilizedinmanyfields,particularlyintheareaofbiochemicalanalysis．Manybio-analysisaboutnucleicacids，amineacid，proteins，cellsandtoxinbymicrofluidicstechnologyhavebeenreported．Atthesametime，Poll

4、utionoftheenvironmenthasreceivedunprecedentedattention．Themonitoringanddetectionofthepollutionisthebasicworktorepaktheenvironn℃nLEffectivedetectiontechnologycanpmvideslrongsupportforenvironmentalpollutionandprevention,andalsoprovideevidencesandet＆tiveassessmentforpublichealthandsaf

5、ety．Therearemanymaturetechnobgiestodetectandmonitorenvironmentalpollutants．Forthedetectionofatmosphericpollutants，methodssuchasGaschromatography,theinfraredabsorption,eleclrochemicalanalysisareallqualified．Andasforpollutantsinthewater,wecanUsechromatographicdetectionmethod，immunolo

6、gy,andbiobgicaldetectionmethod．Allthesetechnobgieshavegoodprecisionandacctracy,butmostofthemaleexpensive，andneedeitherlonganalysisperiodorcomplexoperation,notsuitableforon-sitedetection．Thereisagreatneedtodevebpaportable，high—throughputandsensitivemethodforrapiddetectionofpollutant

7、．Nowadays，thecombinationofmicrofluidiechipsandthedetectionofpollutantsappealmoreandmoreattention．Andinthispaper,wesuccessfullybuilttheimmunoreactionsplatformonmicrofludicchips,andutilizedtodeveloptherapiddetectiontechnobgyforcotIlilleandglyphosate．111tl℃fastpart,amicrofluidicimmuno

8、assayissuccessfullydevelop