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1、SCI.MAR.,64(2):235-246SCIENTIAMARINA2000AQUATICFLOWCYTOMETRY:ACHIEVEMENTSANDPROSPECTS.M.RECKERMANNandF.COLIJN(eds.)Flowsortinginaquaticecology*MARCUSRECKERMANNResearch-andTechnologyCentreWestcoast(FTZ)ofKielUniversityD-25761Büsum.E-mail:recker@ftz-west.uni-kiel.deSUMMARY:Flowsortingcanbeaveryhelpfu
2、ltoolinrevealingphytoplanktonandbacterialcommunitystructureandelaboratingspecificphysiologicalparametersofisolatedspecies.Dropletsortinghasbeenthemostcommontechnique.Despitethehighopticalandhydro-dynamicstressforthecellstobesorted,manyspeciesgrowinculturesubsequenttosort-ing.Todate,flowsortinghasbe
3、enappliedtopost-incubationseparationinnaturalwatersamplestoaccountforgroup-spe-cificphysiologicalparameters(radiotracer-uptakerates),totheproductionofclonalornon-clonalculturesfrommixtures,totheisolatonofcellgroupsfromnaturalassemblagesformolecularanalyses,andfortaxonomicidentificationofsortedcells
4、bymicroscopy.TheapplicationofcellsortingfromnaturalwatersamplesfromtheWaddenSea,includingdifferentcryptophytes,cyanobacteriaanddiatoms,isshown,aswellastheestablishmentoflaboratoryculturesfromfieldsamples.Theoptionaluseofaredlasertoaccountforphycocyanine-richcellsisalsodiscussed.Keywords:flowcytomet
5、ry,flowsorting,phytoplankton,bacteria,cultures.INTRODUCTIONingidenticalorsimilaropticalpropertiesappearasdistinctclustersinatwo-parameterhistogramordotTheessenceofflowcytometryisthesimultane-plot,whicharemoreorlessseparatedfromeachousmeasurementofdifferentopticalcellpropertiesother.Flowsortingtakes
6、this“virtual”separation(lightscatterandmulticolourfluorescenceemis-onestepfurther,byphysicallyseparatingthecellssion),whichallowthecharacterizationandclassifi-fromthemixedassemblage.Sincetheearlydaysofcationofindividualcellsinamixture(Hofstraatetcommerciallyavailableflowcytometers,sortinghasal.,199
7、1;VeldhuisandKraay,2000).Theaquaticbeenaroutinefeatureofmanyinstruments.scienceshavestartedtomakeuseofflowcytome-Theidentificationanddifferentiationofmalig-tersinthelate1970s(Paauetal.,1978).Theauto-nantblo
