xrcc1对脑胶质瘤细胞生物学行为影响的分析

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1、徐州医学院硕士学位论文关键词XRCCI；胶质癯；迁移；侵袭；增殖；凋亡；周期4徐州医学院硕士学位论文EffectsofXRCC1onbiologicalbehavioringliomacellsAbstractObjectiveTheaimofthisstudyistoinvestigatetheroleofXRCC1(X-rayrepaircross-complementinggroup1)ingliomacellsgrowth，migration，invasion，cycleandapoptosis，anditCallprovideexperimentalbasisfort

2、heinvolvedmolecularmechanismofglioma．MethodsWeuseControlsiRNA、XRCC1siRNA；Controloverexpressionplasmid、XR．CCIoverexpressionplasmidrespectivelytotransfectgliomacellsU87andU251．Then，theexpressionlevelsofXRCCIingliomacellsaredetectedbywesternblot．TheinfluenceontheproliferationofgliomacellsU87、U2

3、51cellaremeasuredbyCCK-8assay．TheinfluenceonthemigrationandinvasionofgliomacellsU87andU251cellaremeasuredbyTranswellassay．TheinfluenceonthecycleandapoptosisofgliomacellsU87andU251cellareanalyzedbyflowcytometry(FCM)．Results1．TocompareControlsiRNAgroupwithXRCC1siRNAgroup，thelattercouldknockdow

4、ntheexpressionofXRCCI，coulddrasticallyincreaseODvaluesofcellproliferationin72hand96hbyCCK-8assay,increasetheabilityofcellmigrationandinvasion，increasethecellsproportioninthephaseSandG2／MingliomacellsU87andU251，anddecreasetheapoptosisrateofU251cellsbyFCM．Differenceswerestatisticallysignifican

5、t(P<0．05)．WhiletherewasnodifferenceabouttheapoptosisrateofU87cells(P>0．05)．2．TocompareControloverexpressionplasmidgroupwithXRCC1overexpressionplasmidgroup，thelattercouldincreasetheexpressionofXRCC1，coulddrasticallydecreaseODvaluesofcellproliferationin72hand96hbyCCK-8assay，decreasetheabilityo

6、fcellmigrationandinvasion，decreasetheceilsproportioninthephaseSandG2／MingliomacellsU87andU251，andincreasetheapoptosisrateofU251cellsbyFCM．Differenceswerestatisticallysignificant(P<0．05)．WhiletherewasnodifferenceabouttheapoptosisrateofU87cells(P>0．05)．Conclusions1．TheexpressionlevelofXRCCIinh

7、umangliomacellscouldinfluencebiologicalbehavior,whichsuggeststhatXRCC1iscloselyrelatedtogliomadevelopment．2．XRCC1couldinhibittheabilityofcellmigrationandinvasionofU251andU87gliomacelllines．3．XRCCImightinhibitgrowthofU251andU87gliomacelllinesbymedia