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1、2期汤绍虎等：外源NO对渗透胁迫下黄瓜种子萌发、幼苗生长和生理特性的影响421外源NO对渗透胁迫下黄瓜种子萌发、幼苗生长和生理特性的影响汤绍虎，周启贵，孙敏，毛薇（西南大学生命科学学院，重庆400715）摘要：【目的】探究外源NO对渗透胁迫下黄瓜（CucumissativusL.）种子萌发、幼苗生长和生理特性的作用。【方法】在25%PEG-6000胁迫下，研究0.1和0.5mmol·L-1SNP（硝普钠）对黄瓜种子萌发、幼苗生长、叶片氧化损伤和保护酶活性的影响。【结果】0.1mmol·L-1SNP处理能显著缓解25%PEG-6000的胁迫伤害，使黄瓜种子萌发率提高0.24倍，
2、发芽指数提高0.86倍，活力指数提高3.01倍；幼苗株高、根长和干重分别提高1.15，2.17和1.16倍；叶片Pro含量提高50.99%，SOD，CAT，POD和APX活性分别提高42.39%，66.58%，79.03%和116.39%，MDA含量降低80.50%。与0.1mmol·L-1SNP处理相比，0.5mmol·L-1SNP处理促进黄瓜种子萌发和幼苗生长，缓解叶片氧化损伤和提高保护酶活性的作用明显或显著降低。【结论】0.1mmol·L-1SNP能显著促进渗透胁迫下黄瓜种子萌发和幼苗生长，明显缓解叶片氧化损伤，显著提高SOD等保护酶活性；0.5mmol·L-1SNP的
3、有效作用减弱，甚至抑制SOD活性。关键词：黄瓜；种子萌发；一氧化氮（NO）；渗透胁迫；氧化损伤EffectsofExogenousNitricOxideonSeedGermination,SeedlingGrowthandPhysiologicalCharacteristicsofCucumberUnderOsmoticPressureTANGShao-hu,ZHOUQi-gui,SUNMin,MAOWei(CollegeofLifeScience,SouthwestUniversity,Chongqing400715)Abstract:【Objective】Theobjec
4、tiveofthisstudywastoinvestigatetheeffectsofexogenousnitricoxideonseedgermination,seedlinggrowth,andphysiologicalcharacteristicsinosmoticallystressedcucumber(CucumissativusL.).【Method】Weused25%PEG-6000toproducetheosmoticpressure,underwhichtheeffectsofsodiumnitroprusside(SNP),anexogenousnitri
5、coxidedonor,weremeasuredonseedgermination,seedlinggrowth,oxidativedamagetoleaves,andprotectiveenzymeactivitiesincucumber.WecarriedouttheaforementionedexperimentsattwoconcentrationsofSNP:0.1and0.5mmol·L-1.【Result】Thedamagetocucumbercausedbytheosmoticstressof25%PEG-6000wassignificantlyreduced
6、inthepresenceof0.1mmol·L-1ofSNP.Specifically,theseedgerminationrate,germinationindex,andvigorindexincreasedby0.24-fold,0.86-fold,and3.01-fold,respectively;plantheight,rootlength,anddryweightincreasedby1.15-fold,2.17-fold,and1.16-fold,respectively;prolinecontentandenzymeactivitiesofSOD,POD,C
7、AT,andAPXinleavesincreasedby50.99%,42.39%,66.58%,79.03%,and116.39%,respectively;thelevelofmalondialdehyde(MDA)inleavesdecreasedby80.50%.WhentheconcentrationofSNPwasincreasedto0.5mmol·L-1,weobservedadiminishingstimulativeeffectoncucumberseedgerminationand
