卢氏绿壳蛋鸡蛋壳腺中胆绿素时空变化及鸡蛋品质的变化

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1、河南农业大学硕士学位论文卢氏绿壳蛋鸡蛋壳腺中胆绿素时空变化及鸡蛋品质的变化姓名：张学武申请学位级别：硕士专业：基础兽医学指导教师：李奎；康相涛201206河南农业大学牧医工程学院2012届毕业研究生摘要本文运用人体解剖学、组织化学技术、分子生物学测定22．31周龄卢氏绿壳蛋鸡鸡蛋壳中胆绿素含量、鸡蛋品质、壳腺部绒毛高度、壳腺部胆绿素含量、壳腺部血红素加氧酶．1的mRNA相对表达量，米研究不同周龄卢氏绿壳蛋鸡壳腺部胆绿素时空变化与蛋品质的关系，为卢氏绿壳蛋鸡的生产养殖提供基础理论。结果显示：(1)卢氏绿壳蛋鸡绿壳蛋蛋壳中H日绿素含量先降低斤增加再降低的波动变化。第22周蟹壳中胆绿

2、素含量高丁第25周，著异显著。第28周月日-绿素含量高丁第25周，筹异显著。(2)在22—31周龄，卢氏绿壳鸡蛋品质随着周龄的增加而增人。蛋黄重逐渐增加，差异显著。(3)在22-31周龄，卢氏卢氏绿壳蛋鸡壳腺部绒毛高度逐渐增加，第22周与第25周、28周、31周著异显著。(4)卢氏绿壳蛋鸡壳腺部胆绿素含量在第22周至第31周，壳腺部胆绿素含量逐渐增加，第22周与第25周、第28周、第31周著异显著。(5)卢氏绿壳蛋鸡壳腺部血．红素加氧酶．1的mRNA相对表达量是先增加后减少再稳定的变化趋势，第25周血．红素加氧酶．1的mRNA相对表达量高丁．第22周、第28周、第31周，筹异显

3、著。关键词：卢氏纯绿壳蛋鸡；壳腺部；胆绿素；鸡蛋品质；血红素加氧酶．1ThespatialtemporalchangesofbiliverdinintheshellglandandthechangesofeggqualityofGreen—EggshellofLushiChickenABSTRACT：TostudytherelationshipbetweenthespatialtemporalchangesofbiliverdinintheshellglandandtheeggqualityofGreen—EggshellofLushiChickenandDrovidetheo

4、riesbasisforproductionandfarmingofLushiGreen。EggshellChicken．weusedtheanatomytechnology、histochemistrytechnology、Molecularbi010坚vtecllniquestodetectethebiliverdincontentingreeneggshell、Eggquality、villiheightofgreenshellhenseggshellgland、Thebiliverdincontentofgreenshellhenseggshellgland、theHO

5、一1mRNARelativeexpressionineggshellglandofgreenegg．shellofLushiChickenin22weeksto31weeks．Theresultsshowedthat：(1)ThebiliverdincontentofGreenshelleggseggshellinGreen—EggshellofLushiChickenpresentedfirstdecreasesandthenincreasesandthendecreasesasthefluctuation．Thebiliverdincontentintheeggshelli

6、ntwenty—secondweekswashigherthanthatintwenty—fifthweeks：andthedifferencesbetweenthenweresignificant．Thebiliverdincontentintheeggshellintwenty—eighthweekswashigherthanthatintwenty—fifthweeks．andthediffere：ricesbetweenthenweresignificant·(2)Inthe22．31weekageeggqualityoftheGreen—EggshellofLushi

7、Chickenincreasedastheweekage．Yolkweightincreasedgradually，andtherewasasignificantdifferencebetweenthem．(3)Inthe22．31weekagethevillusheightoftheeggshellinGreen—EggshellofLushiChickenincreasedgradually,andthevillusheightintwenty。secondweekshadasignif