short term outcome of human fetal ovarian tissue cryopreservation and xenotransplantation to immunodefficient mice论文

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1、MASTER’sDEGREETHESISSHORTTERMOUTCOMEOFHUMANFETALOVARIANTISSUECRYOPRESERVATIONANDXENOTRANSPLANTATIONTOIMMUNODEFFICIENTMICE.ATHESISSUBMITTEDTOTHEDEPARTMENTOFOBSTETRICSANDGYNECOLOGY,WUHANUNIONHOSPITAL,TONGJIMEDICALCOLLEGEHUAZHONGUNIVERSITYOFSCIENCEANDTECHNOLOGYW

2、UHAN430030,PRCHINABYAMADUSESAYINPARTIALFULFILLMENTOFTHEREQUIREMENTSFORTHEDEGREEOFMASTER’SINOBSTETRICSANDGYNECOLOGYSUPERVISOR:PROF.WANGHONGBOTH10APRIL2012ListofAbbreviationsandAcronyms:BRCA–BreastCancerAntigenCD-ClusterofDifferentiationDAB-DiaminobenzeneDMSO-D

3、imethylsulphoxideFSH-FollicleStimulatingHormoneHCl-HydrochloricacidHLA–HumanLeukocyteantigenHSA–HumanSerumAlbuminHSCT-HematopoieticStemCellTransplantationIP-IntraperitonealIU-InternationalUnitsL15-Leibovitz15MediumMHC–MajorHistocompatibilityComplexOB-OvarianB

4、ursaOT-OvariantissueOTC-OvarianTissueCryopreservationPBS-PhosphateBufferedSalinePOF-PrematureOvarianFailureSC-SubcutaneousSCID-SevereCombinedImmunoDefficiencyTBST-TrisBufferedSalineTweenXNA-XenoreactiveNaturalAntibodyiTableOfContentsListOfAbbreviationsAndAcro

5、nyms…………………….………………………………..…iTableOfContents………………………………………………………………………………iiAbstract………………..……………………………………………………………………......ivCHAPTER1BACKGROUNDANDLITERATUREREVIEW1.1BackgroundAndLiteratureReview…………………………………………………..….11.2Objective………………..……………………………………………………

6、…………..…61.3Setting..…………………………………………………………………………………...…7CHAPTER2MATERIALSANDMETHODS2.1DesignofExperiment………….………………………………………………………...….92.2Experimentalanimals…………………………………………………….……......102.3HarvestingOfHumanFetalOvarianTissue………………………………………..….....112.4Free

7、zingAndThawingOfOvarianTissue……………………………….………….…...122.5XenograftingOvarianTissueToMice………………………………………………….....142.6PostoperativeMouseObservationandTreatment…………………….………………..….162.7EuthanasiaAndGraftRecovery……………………………………………………...…..162.8HLA-DRstainingoffetal

8、andadultovariantissues......................………………..…..172.9HematoxylinandEosinstaining..........…………………………………………..…….....19CHAPTER3RESULTSOFEXPERIMENTSii3.1ImmunhistochemistrystainingRes