资源描述：
《解偶联蛋白2通过线粒体介导参与高血糖加重脑缺血再灌注损伤的实验分析》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、宁夏医科大学硕士研究生论文英文摘要Uncouplingprotein2playaroleinhyperglycemiaaggravatedcerebralischemia-reperfusioninjurymediatedbythemitochondriaABSTRACTObjectiveDiabeteshyperglycemiaaggravatedcerebralischemia-reperfusioninjury,andpromotedproductionofmitochondrialreactiveoxygenspecies(ROS)afterischemia.Uncouplingpr
2、otein2(UCP2)canstabilizedthemitochondrialmembranepotentialandreducedthegenerationofROS.Butatpresent,mechanismofactionandregulatorypathwayofUCP2inhyperglycemiaaggravatedcerebralischemiainjuryarenotclear.ThisstudyusingUCP2knockoutmicesimulatedcerebralischemiaandhyperglycemia，toexploreifUCP2knockoutca
3、naggravatedneuronalinjuryanditsmolecularpathways.MethodsMaleUCP2knockoutmice(KO-UCP2)andwild-typeC57BL/6jmicewererandomlydividedintowild-typemicewithnormoglycemicfocalcerebralI/Rgroup(CNgroup),KO-UCP2micewithnormoglycemicfocalcerebralI/Rgroup(KNgroup)andKO-UCP2micewithdiabetichyperglycemicI/Rgroup(
4、KHgroup).AtypeⅠdiabeticratmodelwasinducedbystreptozotocin(STZ)injectionandthefocalcerebralI/Rmodelwasinducedby1hourofmiddlecerebralarteryocclusion(MCAO)followedby6h,24h,72hofreperfusion.Histopa-thology,immunohistochemistry,immunofluorescence,TTCstaining,TUNEL,RT-PCRandwesternblotwereappliedtodetect
5、changesofthemorphologyandthenumberofneurons,apoptosisandtheexpressionofCytC,CleavedCaspase-9,Caspase-3fromeachgroup.Results(1)At24hofI/R,thebrainfunctionscoresofKNgroupwerehigherthanCNgroup（P＜0.05）,andKHgroupwereobviouslyhigherthanKNgroupIII万方数据宁夏医科大学硕士研究生论文英文摘要（P＜0.05）.(2)TTCstainingresultsshownno
6、significantinfarctioninshamgroup.At24hofI/R,infarctsizeofKNgroupwassignificantlylargerthantheCNgroup,andKHgroupwaslargerthantheKNgroup(P＜0.05).(3)Brainedemaandpartialshrinkageofneuronswereobservedininfarctionareaat6hourI/Rafter1hofischemiaandworseddamage,structuralfailure,disorderarrangementat24hou
7、rofI/R.At72h,necroticnervecellsheddingandappearedInflammatorycellsandalargenumberofglialcellsininfarctionarea.ComparedwithCN,KNgroup,evenworsecerebraledemawasdetectedinKHgroup.Aroundtheinfarctionarea,penumb
