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1、症及内皮血管的损伤,有很好的改善作用。关键词:柿叶黄酮脑缺血脑缺血耐受2摘要EffectsofflavonefromleavesofDiospyroskakionischemiatolerancemodelWanglinan(Pharmacology)DirectedbyMiaomingsanAbstractObjective:ToobservetheeffctsflavonefromtheleavesofDiospyroskakionbrainischemiatolerancemodel.Dete
2、rminatethecontentofET,t-PA,PAI-1,TM,vWFintheplasmaofbrainischemiatoleranceanimals,thecontentofTXB2,6-Keto-PGF1αincerebralcortexofmouseandtheexpressionICAM-1ofratbrainhistomorphology.Observethechangesofrelatedpathlogy.Therebyinvestingtheendogenesisprote
3、ctmechanismofischemicpreconditioning,thetherapeuticactionandmechanismofflavonefromtheleavesofDiospyroskakionbrainischemiadisease.Methods:Tieupthebilateralcommonsrteriestwicetobethemodelofmouseischemictolerance.Afterthefirstoperation,everymouseshouldbeg
4、iventheralateddrugs(ginkgobinkgoextract,low,middleandhighdoseofflavonefromtheleavesofDiospyroskaki).Themouseintheshamoperationgroup,bilateralcommoncarotidarteriesgroupandmodelgroupweregivenNSfivedays,onceaday.Afterfasting12handgivingthedrugs,blockthefl
5、owofbilateralcommonsrteries,after30min,recoverytheflow.After24hofthesecondoperation,determinethecontentoft-PAandPAI-1intheplasma,thecontentofTMand6-Keto-PGF1αincerebralcortexofmouse.Observethechangesofmousebrainhistomorphologybymicroscope.Firstblockthe
6、flowofbilateralcommonsrteries,thenusethemethodofreformingLongamethodtomakeratischemictolerance.Afterthefirstoperation,everymouseshouldbegiventheralateddrugs(ginkgobinkgoextract,low,middleandhighdoseofflavonefromtheleavesofDiospyroskaki).Themouseinthesh
7、amoperationgroup,bilateralcommoncarotidarteriesgroupandmodelgroupweregivenNSfivedays,onceaday.Afterfasting12handgivingthedrugs,blocktheflowofmiddlecerebralartery,after2hrecoverytheflow.After24hofthesecondoperation,determinethecontentofET,TMandVwfinthep
8、lasmaandtheexpressionofratbrainhistomorphologybymicroscope.Results:Ischemiatolerancemiceandratsmodelhavebeencopiedsuccessfully.The3柿叶黄酮对脑缺血耐受动物模型的影响t-PAintheplasmaand6-Keto-PGF1αincerebralcortexdecreasesignificantlyofanimalsinthebilater